牛胰多肽与CL／PC脂质体作用后二级结构的变化  

THE SECONDARY STRUCTURE OF BPP AFITR INTERACTION WITH CL/PC VESICLES BY FT-IR SPECTROSCOPY

作　　者：聂松青[1] 杨丽[1] 林克椿[1] 姜厚理[1] 宋占军[1] 

机构地区：[1]北京医科大学生物物理系,军事医学科学院国家生物医学分析中心

出　　处：《生物物理学报》1995年第3期346-350,共5页Acta Biophysica Sinica

基　　金：国家自然科学基金

摘　　要：用傅立叶变换红外光谱（ＦＴ－ＩＲ）研究了ＢＰＰ与膜作用后其二级结构的变化。通过对红外光谱中酰胺Ｉ谱带进行解卷积、微分双曲线拟合等处理，结果表明，ＢＰＰ与脂膜作用后，其二级结构中α－螺旋成分增多，无规卷曲成分减少。The inhibition effects of BPP on the fusion of CL/PC liposomes induced by Ca2+ has been illustrated before. In order to investigate the relationship between the structure and the function of BPP, FT-IR spectroscopy-a recently developed teChnique in studying the secondary structure of proteins and polypeptides was used. After the tmetments of derenvolution. derivation and curve fitting to the able I band, the results revealed that the smict￣ of BPP varied when it inteMcted with the membrane, i. e. the amount of α-helicesincreased, and the proportion of the different kinds of β-structure changed.

关 键 词：牛胰多肽 二级结构 心磷脂 磷脂酰胆碱 脂质体 

分 类 号：Q516.01[生物学—生物化学]