海生植物叶绿体膜中阳离子诱导激发能分配变化的静电控制与非静电控制的研究  

STUDY ON THE ELECTROSTATIC CONTROL AND THE NON-ELECTROSTATIC CONTROL OF CATION-INDUCED EXCITATION ENERGY DISTRIBUTION CHANGES IN THE CHLOROPLAST MEMBRANES FROM MARINE PLANTS

作　　者：高振泮[1] 娄清香[1] 马红[1] 翟小京[1] 马桂芝[1] 毛大璋[1] 李良璧[1] 

机构地区：[1]中国科学院海洋研究所,中国科学院植物研究所

出　　处：《生物物理学报》1995年第4期502-510,共9页Acta Biophysica Sinica

基　　金：国家自然科学基金;EMBL的资助

摘　　要：用Ｃａ２＋和胰酶处理大叶藻（Ｚｏｓｔｅｒａｍａｒｉｎａ）和刺松藻（Ｃｏｄｉｕｍｆｒａｇｉｌｅ）叶绿体膜，研究了它们的类囊体膜多肽组分与Ｍｇ２＋诱导Ｃｈｌａ荧光和膜表面电荷变化之间的相互关系。观察到：（１）在大叶藻的叶绿体膜中，Ｍｇ２＋诱导ＰＳ－Ⅱ荧光强度的增高与其诱导类囊体膜表面电荷密度的降低密切相关；但这种相关性的效应不存在于刺松藻的叶绿体膜中。（２）用Ｃａ２＋处理这两种叶绿体膜分别除去其类囊体膜表面的３２－３４ＫＤ和３０－３１ＫＤ多肽，对上述Ｍｇ２＋诱导的现象无明显的影响。（３）用胰酶进一步消化这些Ｃａ２＋处理过的叶绿体膜分别除去其类囊体膜表面的２６ＫＤ和２３－２４ＫＤ多肽，那么在大叶藻的叶绿体膜中，Ｍｇ２＋诱导荧光和类囊体膜表面电荷变化的相关性效应则全部消失；但在刺松藻的叶绿体膜中，Ｍｇ２＋诱导荧光增高的效应完全消失，而Ｍｇ２＋诱导膜表面电荷变化的性质则保持不变。这些实验结果不仅证明，类囊体膜表面的２６ＫＤ和２３－２４ＫＤ多肽分别为大叶藻和刺松藻叶绿体膜中阳离子诱导激发能在ＰＳ－Ⅱ和ＰＳ－Ⅰ之间分配变化的特异性作用部位；而且说明阳离子调节激发能在这两个光系统之间分配的机理，在这两种海生植物的叶绿体膜中?The interrelations of thylakoid polypeptide components to Mg2+-induced Chlafluorescence and thylakoid surface charge changes were investigated in Zostera marinaand Codium fragile chloroplasts treatal with Ca2+ and trypsin. It was observed that:1), increase of Mg2+-induced PS-Ⅱ fluorescence intensity was closely relatedto Mg2+-induced electric charge density decrease of thylakoid surface in Zostera marinachloroplasts, while such a corralation did not exist in Codium fragile chloroplasts, 2), thetratment of there two konds of chloroplasts with Ca2+ to remove respectively 32-34KD and30-31Kd polypeptides of thylakoid surface did not affect the Mg2+ -induced phenomena;3), when the Ca2+ -treated chloroplasts were further digested by trypsin to remove respectively26KD and 23-24KD polypoptides of the membrane surface, the correlative effect ofMg2+-induced fluorescence and thylakoid surface charge changes would completely disappearin the Zotera marina chloroplast; but in the Codium fragile chioroplast, the canon-inducedfluorescence increase effect would Wholly eliminate Wheteas the property of the cation-inducedsurface charge changes of the membrane remain unchanged. These results not only show thatthe 26KD and 23-24KD polypeptides on the thylakoid surface are the specific acting sites ofthe cation that induce Call fluorescence changes in the chloroplasts from these two species ofmarine plants respectively, but also demonstrate that the mechanism of the regulating effect ofcanon on excitation energy distribution between PS-Ⅱ and PS-Ⅰ in Zostera marinachloroplast is quite different from that in Codium flagile chloroplast. The former is controlledby the electrostatic property of the thylakoid surface while the latter does not.

关 键 词：海生植物 藻类 叶绿体膜 光系统 激发能的分配 

分 类 号：Q949.207[生物学—植物学]