大鼠输精管注射HFMC后输精管内pH值的观察  

Studies on pH of Internal Vas Deferens after Intra-vas Injection of HFMC foe Contraception in S. D. Rats

作　　者：何亚平[1] 岳利民[1] 罗自强[1] 张金虎[1] 陈新汉 

机构地区：[1]华西医科大学基础医学院

出　　处：《生物医学工程学杂志》1995年第4期281-283,共3页Journal of Biomedical Engineering

摘　　要：本实验选用生育力正常成年雄性ＳＤ大鼠２５只，随机分为５组，行一侧输精管ＨＦＭＣ注射术，剂量为１０％ＨＦＭＣ２０μｌ（２ｍｇ），沉淀剂２０μｌ；另一侧输精管注射生理盐水４０μｌ对照，于注射时和注射后１、３、６、９和１２月分别测定双侧输精管ｐＨ值，其结果为注射ＨＦＭＣ侧输精管ｐＨ明显降低，为５．１８～５．８２，生理盐水对照侧为７．０４～７．６４，并随时间推移，注射ＨＦＭＣ侧输精管ｐＨ值逐渐升高，于术后１２月基本恢复与对照侧基本相同。提示：输精管注射ＨＦＭＣ后，能造成输精管内低ｐＨ值，改变了生殖系统内环境，影响精子功能，这可能是输精管内注射ＨＦＭＣ节育的机理之一。pH of internal vas deferens was measured by PH meter (pHs-2type) with micro ion selected electrode. The surgically exposed vas deferens of adult 25 S. D. rats were injected, under visual inspection, withHFMC into the experimental vas on the identical side (dose: 10% 20μl. 2mg) and with sterile filtrated distilledwater in the same dose into the control vas on the opposite side. On both sides, intra-vas PH measurement wasmade at months 0, 1, 3, 6, 9, and 12 with insertion of a micro electrode. The results revealed that pH of the experimental vas was lower than that of control vas by about 2. 5 scales. As the months went on, the pH elevatedgradually to the PH level of the control vas at 12th month. It is evident that HFMC is a kind of hydrogel that releases H+ slowly and maintains the lower PH in vas for a period of time. As a result, the intra-vasal HFMC occludes the vas deferens lumen, releases H+ and thus compels reversal flow to intervene the epididymal milieu andto disturb the epididymal spermatozoa maturation process and consequently and their fertilizing capacity. No sooner had HFMC been progressively dissolved and excreted than the PH returned to normal. Then the fertilizing capacity recovered naturally.

关 键 词：输精管 PH值 高分子聚合物 男性节育 

分 类 号：R169.41[医药卫生—公共卫生与预防医学]