溶脲脲原体感染动物模型的建立─对睾丸的形态学影响  

Establishment of an Animal Model Infected by Ureaplasma Urealyticum ──Its Effects on the Morphology of the Testis

作　　者：徐晨[1] 孙广芳[1] 徐胜[1] 王一飞[1] 

机构地区：[1]上海第二医科大学组织胚胎学教研室

出　　处：《生殖与避孕》1995年第3期205-209,共5页Reproduction and Contraception

基　　金：上海市高教局重点学科基金;国家自然科学基金

摘　　要：本文观察了溶脲脲原体（Ｕｒｅａｐｌａｓｍａｕｒｅａｌｙｔｉｃｕｍ，Ｕ．Ｕ．）对ＳＤ雄性大鼠睾丸的形态学影响。结果表明，在４０例膀胱内接种Ｕ．Ｕ．（１０５ＣＣＵ／ｍｌ）的大鼠中，有２５例睾丸中分离出Ｕ．Ｕ，动物睾丸重量减轻。光镜下可见１１例动物呈广泛的曲精小管萎缩，生精细胞严重脱落。部分曲精小管仅见支持细胞和少量精原细胞，有的仅剩下界膜。在曲精小管中发现许多多核巨细胞，有多核巨细胞处，生精上皮破坏更为明显，间质渗出、水肿。电镜观察发现，Ｕ．Ｕ．可吸附或侵入生精细胞内，造成生精细胞胞浆空泡化乃至崩解，部分线粒体基质空泡化或嵴消失。其余１４只大鼠睾丸组织有局灶性破坏。This study observed the morphologic effects of Ureaplasma urealyticum(U, U.)on the testes of male S. D. rats.It has been shown that of the 40 cases of rats,which were inoculated U. U. (105 CCU/ml)by injecting broth into the urinary bladder following laparectomy, 25 cases were detected U. U. in the testes and the weight of their testes was much lighter. Among the infected animals, 11 cases showed extensive degeneration in seminiferous tubules,germ cells exfoliation and the interstitial tissue permeation and edema. In part of the seminiferous tubules, only the Sertoli cells and few spermatogonia were still present but other germ cells were absent. In some seminiferous tubules,only tunica propria left, many multinucleated giant cells were found in the seminferous tubules,moreover,in these places the germinal epithelium were destroyed more dramatically.Electron microscopic studies revealed that U. U. can attach on or invade into the germ cells and cause the cytoplasm vacuoled and even collapsed. Mitochondria matrix vacuolization or cristae alteration were also found.Local degeneration of testicular tissue was found in the rest of 14 cases of rats.

关 键 词：溶脲脲原体 感染 精子发生 动物模型 大鼠 

分 类 号：R698.202[医药卫生—泌尿科学]