两种杂交群（BALB／c♂×不同♀系）与BALB／c小鼠繁殖性能及单抗腹水产量的比较  

Comparison of Generative Ability and Monoclonal Antibodies Producion in Ascites Between Two Hybrid Strains and BALB/c Mice

作　　者：江其辉[1] 耿志贤[1] 刘宝兴[1] 祝庆余[1] 李钟铎[1] 辛颜彬[1] 魏之玲 

机构地区：[1]军事医学科学院实验动物中心,军事医学科学院微生物学流行病学研究所

出　　处：《实验动物科学与管理》1995年第4期34-37,共4页Laboratory Animal Science & Administration

摘　　要：对两种杂交群（ＢＡＬＢ／ｃ♂×ＫＭ，ＢＡＬＢ／ｃ♂×ＬＡＣＡ♀）的繁殖能力及制备单抗腹水的产量同ＢＡＬＢ／ｃ近交系进行比较。实验表明，两杂交群的各项繁殖指标均优于ＢＡＬＢ／ｃ，总离乳鼠数分别为ＢＡＬＢ／ｃ的１．９８、２．０７倍，接种人肺炎支原体Ｆ８瘤细胞，ｋＣＦ１（ＢＡＬＢ／ｃ×ＫＭＦ１小鼠）的腹水产量为ＢＡＬＢ／ｃ的２．５倍，效价相同；制备六种单抗腹水ＬＣＦ１（ＢＡＬＢ／ｃ×ＬＡＣＡＦ１）的产量均优于ＢＡＬＢ／ｃ，分别为ＢＡＬＢ／ｃ的１．１７～２．８８倍，除Ｅ１０抗体滴度低外，其它各抗体效价相同或接近于ＢＡＬＢ／Ｃ。The generative ability and monoclonal antibodies production in ascites be-tween two hybri strains(BALB/ c♂×KM♀, BALB/ c♂×LACA)and BALB/ cmice were compared。 The experiments show that all results of both bybrid strains in genera-tion are better than BALB / c。 The total weaners of two hvbrid strains are l.98 and 2.07 times as many as BALB / c。The ascites production of(BA LB / c × KM) KCF1 in antimycoplasma pneumoniae is 2.5 times the volume of BALB / c and both titers are equal。The ascites production off(BALB / c♂×LACA) LCF1 in 6 species antibodies are all higher than BALB / c。They are l.17──2.88 times as much as BALB / c individually,and the titers are equal or close to the titers of BALB / c except E10。

关 键 词：实验动物 繁殖 杂交群 单克隆抗体 腹水 制备 

分 类 号：R392-33[医药卫生—免疫学] S865.193[医药卫生—基础医学]