荧光晕法在细胞染色质结构状态检测中的应用  

THE APPLICATION OF HALO ASSAY IN ANALYSIS OF CELL CHROMATIN STRUCTURE

作　　者：罗瑛[1] 孙志贤[1] 夏寿萱 

机构地区：[1]北京放射医学研究所,100850

出　　处：《实验生物学报》1995年第1期37-40,共4页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金

摘　　要：荧光晕法是近年来发展起来的定量检测细胞染色质结构状态的方法,由于其简易、直观、灵敏、快速,已被广泛用于放射生物学及肿瘤细胞生物学研究中。我们依据文献报道建立了此法,并对其进行了改良,进一步优化了操作步骤。并用该法检测了两种相同来源(具有相似遗传背景)、具不同辐射敏感性的细胞株的染色质结构状态,发现辐射敏感株SX-9具有更松散的染色质结构,可能与两者的辐射敏感性差异有关,为研究染色质结构状态与辐射敏感性的关系提供了佐证。Halo assay, a method for quntitative analysis of chromatin structure in cell, was developed recently. Due to visualization, feasibility, rapidity, and sensitivity of the method, it has been applicated in radiobiology and cancer research. According to previous reports, with some improvement, this method has been established in our laboratory. Employing halo assay, the cbromatin structure of two kinds of cell strain with similar genetic background and different radiosensitivity was analyzed and the results show that radiosensitive strain has looser chromatin structure than radioresistant one. It is possible that this difference is related to their different radiosensitivity, thus it's a proof for the relation of chromatin structure and radiosensitivity.

关 键 词：染色质 结构 荧光晕法 

分 类 号：Q243[生物学—细胞生物学]