吖啶橙染色法检测间日疟原虫的现场应用  

FIELD APPLICATION OF ACRIDINE ORANGE STAIN FOR DIAGNOSING VIVAX MALARIA

作　　者：朱声华[1] 刘庆[1] 王兴振[1] 雷天 李荣和 

机构地区：[1]华西医科大学寄生虫学教研室,筠连县卫生防疫站,名山县人民医院

出　　处：《实用寄生虫病杂志》1995年第3期117-119,共3页Journal of Practical Parasitic Diseases

基　　金：UNDP/WorldBank/WHO/TDR提供基金

摘　　要：作者等采用改进的荧光光源及普通显微镜，于１９９４年７—８月间分别在四川省筠连县和名山县间日疟流行区，对吖啶橙染色技术检测疟原虫进行了现场应用研究。共检测２１０例门诊就医发热病人，与经典的姬姆萨染色检测结果比较分析，吖啶橙染色法的敏感性为９７．２％，特异性为９１．２％，两种方法的符合率为９４．３％。检测速度可以提高２倍以上，明显降低了基层医院检验人员的工作强度。此种荧光光源结构简单，效能稳定，易于操作，价格较便宜，利于在基层医疗及防疫部门推广使用。Acridine orange (AO) stain using an improved fluorescent light source, interference filter supported by Fumihiko Kawamoto of Japan and conventional light microscope were applied for diagnosing vivax malaria in endemic areas of Junlian and Mingshan counties during July and August, 1994. 210 cases of clinic patients with fever were examined and the results were compared with the Giemsa technique (GS). The sensitivity, specificity and concordance rates of AO stain technique were 97. 2%. 91.2%, and 94.3% respectively. The authors suggested that this kind of fluorescent light source would be practical to be used in local hospitals and health stations because of its simplicity, stability, low price and easy manipulations.

关 键 词：疟疾 吖啶橙染色法 间日疟原虫 病原学诊断 

分 类 号：R531.304[医药卫生—内科学]