联合检测循环抗原和抗体在血吸虫病基本控制地区应用价值的研究  

DETECTION OF CIRCULATING ANTIGEN AND ANTIBODY IN SCHISTOSOMIASIS BASICALLY CONTROLLED AREAS

作　　者：张素娥[1] 汤益[1] 施晓华[1] 程国强[1] 郁俊豪 屠乐鸣 沈忠伟 

机构地区：[1]浙江省医学科学院寄生虫病研究所,嘉兴市地方病防治所

出　　处：《实用寄生虫病杂志》1995年第4期161-163,共3页Journal of Practical Parasitic Diseases

摘　　要：血吸虫病基本控制地区７岁以上的人群共８４７名为检测对象，应用抗日本血吸虫ＣＣＡ单克隆抗体ⅢＤ１０建立的Ｄｅｔ－ＥＬＩＳＡ检测血吸虫循环抗原（ＣＡｇ），ＥＬＩＳＡ和ＩＨＡ检测循环抗体（ＣＡｂ）。结果ＣＡｇ的阳性检出率为３．１９％，ＥＬＩＳＡ和ＩＨＡ检测ＣＡｂ的阳性检出率分别为６．２６％和９．４５％，两者的检出车间差异有显著性（Ｐ＜０．０１），ＣＡｇ与ＣＡｂ（ＥＬＩＳＡ和ＩＨＡ检测）的联合检出率分别为６．８５％和１０．５１％，两者联合检测能提高检出率。847 sera specimens from persons over 7 years of age living in schistosomiasis basically controlled areas were examined by monoclonal antibody Ⅲ D10 based Dot-ELISA for detecting circulating antigen (CAg) and by ELISA and IHA for circulating antibody (CAb). The results showed that the positive rate of CAg was 3. 19%, and of CAb by ELISA and IHA were 6. 26% and 9' 45% respectively. These percentages were higher than that of CAg by Dot-ELISA.There was statistically significant difference between positive rates of CAg and CAb (P<0. 01).The combined detection rates of CAg by Dot-ELISA with CAb by ELISA and IHA were 6. 85% and 10' 51% respectively. It is suggested that combined detection of CAg and CAb can raise the detection rate of schistosomiasis.

关 键 词：血吸虫病 循环抗原 循环抗体 联合检测 诊断 

分 类 号：R532.210.4[医药卫生—内科学]