EB病毒转化的人外周血淋巴细胞与SHM—D33细胞系融合制备人单克隆抗体  

Preparation of human monoclonal antibody by hybridization of EB virus transformed peripheral blood lymphocytes with heteromyeloma SHM-D33

作　　者：崔运昌[1] 朱勇[1] 高磊[1] 孙忱[1] 米力[1] 董帮权[1] 

机构地区：[1]第四军医大学人单抗研究组

出　　处：《中国免疫学杂志》1989年第3期133-136,共4页Chinese Journal of Immunology

摘　　要：用EB病毒转化人外周血淋巴细胞,因克隆化不成功,分泌不稳定而失败。将转化的细胞与KR—12细胞融合,因融合率低而未成功。将转化细胞与SHM—D33细胞融合,成功地建立了3株稳定分泌人单抗的杂交瘤细胞,均产生IgMλ型抗体,30～50μg/ml上清。其中1株(86—2)已连续传代18个月,未经克隆化仍稳定分泌抗体,已适应低血清培养,抗体分泌量不变;可用裸鼠制腹水。The hybridization of EBV-transformed PBL (EBV-PBL) with heteromyeloma SHM-D33 and human hybrid myeloma KR-12 were performed. Three human (human-mouse) [H-(H-M)]hybridoma lines secreting specific human monoclonal antibody (HMcAb) at 30-50μg/ml supernatant were established, one of them, 86-2, has been continuously cultured for 18 months without cloning and is still secreting HMcA b. This line was adapted for low-serum growth without reducing of antibody production. The ascites rich in HMcAb were prepared by injecting i. p.the hybridoma cells into NIH nude mice. It is suggested that SHM-D33 is a suitable fusion partner for efficient establishment of H-(H-M) hybridoma producing HMcAb. It was not successful to try to make human-human hybridoma secreting HMcAb by fusion of EBV-PBL with KR-12. We show here that it is a good way to produce stably HMcAb with higher titer by fusion of EBV-PBL with SHM-D33.

关 键 词：EB病毒 杂交瘤 单克隆抗体 

分 类 号：R392.11[医药卫生—免疫学]