PCR用于孕牛外周血中胎儿SRY序列检测和奶牛早期胚胎性别鉴定  

Detection of Bovine Fetal Y-Specific Sry from Preimplanted Embryo Cells and Maternal Blood Using Polymerase Chain Reaction

作　　者：杨建民[1] 王丽山[1] 刘连瑞[1] 蒋耀青 

机构地区：[1]中国科学院遗传研究所

出　　处：《首都师范大学学报（自然科学版）》1995年第2期79-82,共4页Journal of Capital Normal University：Natural Science Edition

摘　　要：根据文献报道的人，鼠，兔ＳＲＹ同源序列设计引物，从牛基因组中扩增出一段牛特异的ＳＲＹ序列，对该序列进行克隆测序后设计出一对雄牛特异的ＰＣＲ引物，利用此引物对奶牛早期胚胎细胞和妊娠晚期母牛外周血ＤＮＡ样品进行ＰＣＲ扩增，经分娩牛犊性别验证，本引物能够准确鉴别孕牛外周血中雄性胎儿的ＳＲＹ序列，并且对早期胚胎性别签定的准确率为８０％．Based on the human, rat and rabbit SRY homologous sequence, a pair of primers was designed to amplify cattle Sry fragment with PCR. With our new male-specific cattle Sry sequence, a pair of primers specific to cattle Sry was developed to sexing preimplanted embryo cells and to detect bovine fetal Sry sequence from maternal peripheral blood. The results showed that bovine Y-specific Sry sequence can be detected directly from preimplanted embryo cells for sex manipulation and from maternal blood for non-invasive identification of fetal sex.

关 键 词：Sry序列 PCR 奶牛 胚胎 性别 鉴定 

分 类 号：S823.3[农业科学—畜牧学]