日本血吸虫感染动物红细胞上非游离性循环抗原水平的检测和动态研究  

Studies on the Detection and Dynamic of Non--free Circulating Antigen on Red Blood Cells of RabbitsIhfected with Schistosoma Japonicum

作　　者：李允鹤[1,2] 陈家旭[1,2] 谢可鸣[1,2] 龚唯[1,2] 夏超明[1,2] 胡永德[1,2] 骆伟[1,2] 

机构地区：[1]苏州医学院寄生虫学教研室 [2]苏州医学院寄生虫学专业

出　　处：《苏州医学院学报》1995年第1期1-3,共3页Acta Academiae Medicinae Suzhou

基　　金："八五"国家攻关课题

摘　　要：应用抗日本血吸虫可溶性虫卵抗原的多克隆抗体（抗ＳＥＡ－ＰｃＡｂ）检测感染５０～２００条尾蚴后不同时间的兔红细胞（ＲＢＣ）上非游离性循环抗原（ＮＦ－ＣＡｇ）。结果表明，感染兔ＲＢＣ上存有ＮＦ－ＣＡｇ，而正常家兔ＲＢＣ上则无；ＧＭＲＴ，前者为２．８３±０．１７～１０２４．００±０．００，后者为１．００±０．００～３．３６±０．１５，两者有显著性差异（均Ｐ＜０．０１）：感染家兔后４～７周内，ＧＭＲＴ呈现低水平，８～９周时显著升高，１０周时均为高峰（ＧＭＲＴ达１０２４．００±０．００），１０周后，均明显下降，１８周时为低水平。结果表明．ＮＦ－ＣＡｇ检测优势仅在感染早期（１０周内）。本文报告感染兔ＲＢＣ上存有ＮＦ－ＣＡｇ，尚未见类似报道，为今后研究ＣＡｇ的新途径和新内容提供了依据，也为感染宿主体液内ＣＡｇ的归宿问题提供了重要依据。he levels of non--free circulating antigen(NF-- CAg) on red blood cells of the rabbits infectedwith 50￣ 200 schistosome cercariae were detected by indirect haemagglutination test (IHA) usingPolyclonal antibody of anti -- SEA (soluble schistosome egg antigen) (anti SEA -- PcAb) at variousperiods after infection. The results obtained from tWO experiments show that NF--CAg are detected onred blood cells of infected rabbits, while not on there of control rabbits and that GMRT ranges from 2.83± 0. 17 to 1024. 00± 0. 00 with the former the from 1. 00± 0. 00 to 3. 36± 0. 15 with the latter.The significant difference(all P values<0. 01) between the both results is available. The low levels(GMRT) of NF--CAg on red blood cells of infected rabbits were found in the 4th to 7th week afterinfection, markedly roce in the 8th to 9th week,and reached peak levels in the 10th week (both are1024. 00± 0. 00),afterwoods, the levels of NF-CAg decreased remarkably after the 10th week,andthe low level showed in the 18th week after infection. The results indicated that the superiority Of NF--CAg detection demonstrated only at the early stage within 10 weeks after infection. The report has not seen as yet. This study provides the important bases for approaching new ways andcontents and understanding the final inhabitation for circulating antigen in the body fluid,namely freeCAg.

关 键 词：兔 红细胞 抗原 日本血吸虫病 多克隆抗体 

分 类 号：R532.210.4[医药卫生—内科学] R446.62[医药卫生—临床医学]