甘草黄酮体抗促癌、抗致突和抗氧化作用的研究  

ANTI-PROMOTING,ANTIMUTAGENIC AND ANTIOXIDANT ACTION OF GLYCYRRHTZAE FLAVONOTDS

作　　者：傅乃武[1] 刘朝阳[1] 张如意[2] 

机构地区：[1]中国医学科学院肿瘤研究所,北京100021 [2]北京医科大学药学院植化室

出　　处：《天然产物研究与开发》1995年第4期29-34,共6页Natural Product Research and Development

摘　　要：G9315是从胀果甘草[Glycyrrhizae inflata Bat(Ⅲ))中提取的含有6个黄酮体的复合物。2mg剂量明显抑制二甲基苯蒽(DMBA)合并巴豆油诱发的小鼠皮肤乳头瘤的生成,抑瘤作用主要在促癌阶段。1mg剂量显著抑制巴豆油诱发的小鼠耳部水肿。小鼠口服G_(9315)(0.5g/kg/d×7)明显对抗环磷酰胺诱发的骨髓微核细胞增加。20—40μg/ml显著抑制TPA促进的^(32)pi参入HeLa细胞的磷脂部分。20μg/ml显著抑制巴豆油诱发的Wistar大鼠中性粒细胞(PMN)和Balb/c新生小鼠皮肤表皮细胞的化学发光以及肝线粒体的脂质过氧化。G(9315)(10—20μg/ml)有效对抗CCl_4诱发的小鼠肝微粒体脂质过氧化。G9315 was a complex extracted from Glycyrrhizae inflata Bat ( Ⅲ ) and consists of 6 flavonoids with significant antioxidant effects. At 1 mg dose it inhibited the mouse ear edema induced by croton oil,and at 2 mg dose it showed strong anti-promoting effect on two stage carcinogenesis in mouse skin induced by DMBA plus croton oil. G9315 (0. 5g/kg/d × 7) markedly inhibited the micronu-clei formation of mouse bone marrow induced by cytoxan (70 mg/kg,ip×1). At 20 μg/ml dose,it inhibited the TPA enhanced 32Pi-incorporation into phospholipid fraction in Hela cells,and the chemilu-minescence (CL) of Wistar rat PMNs and skin epithelial cells of new born Balb/c mouse induced by croton oil were also inhibited. The lipid peroxidation of mouse liver mitochondria induced by croton oil and liver microsome induced by CCl4 were also inhibited by G9315 at the dose of 10-20 μg/ml.

关 键 词：中药 甘草 黄酮体 药理学 抗促癌 抗致突 抗氧化 

分 类 号：R282.710.5[医药卫生—中药学]