LHRH及P物质对大鼠脊神经节细胞膜GABA_A受体介导反应的抑制作用  

Inhibitory Effects of LHRH and Substance P on the Membrane Response Mediated by GABA_A Receptor in Spinal Ganglion Neurons

作　　者：周小萍[1] 关兵才[1] 李之望[1] 

机构地区：[1]武汉同济医科大学实验医学研究中心电生理室

出　　处：《同济医科大学学报》1995年第1期1-4,共4页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金

摘　　要：实验在幼年大鼠脊神经节（ＳＧ）标本进行细胞内记录。结果表明，单独滴加０．０１～１．００ｍｍｏｌ／Ｌγ－氨基丁酸（ＧＡＢＡ）在大多数受检细胞（１０６／１２７）引起荷包牡丹碱敏感的去极化，而胞体膜对０．０１～０．１０ｍｍｏｌ／Ｌ促性腺素释放激素（ＬＨＲＨ）或０．００１～０．０１ｍｍｏｌ／ＬＰ物质（ＳＰ）仅有轻微的去极化或无反应。若将此两种肽类物质在ＧＡＢＡ作用之前分别加至ＳＧ细胞，这时ＧＡＢＡ所引起的反应便大为减弱。结果提示，肽类物质ＬＨＲＨ及ＳＰ对ＧＡＢＡ_Ａ受体介导的初级传入终末突触前抑制具有调制作用。Intracellular recordings were performed on the preparation of immature rat spinal ganglion. In the majority of neurons tested bath application of γ-aminobutyric acid (GABA 0.01～1.00 mmol/L)induced a depolarization, which could be blocked by bicuculline reversibly. Preapplication of LHRH(0.01～0.10 mmol/L)or SP(0.001～0.01mmol/L)attenuated GABA-depolarization markedly, while LHRH or SP applied alone elicited no effect or slight depolarization. The results suggest that peptide LHRH or SP might exert an inhibitory effect on GABA-induced depolarization in the primary sensory neurons.

关 键 词：脊神经节 促性腺激素 P物质 Γ-氨基丁酸 LHRH 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学] Q57[医药卫生—基础医学]