肿瘤体外化疗药物敏感性检测方法的研究  

A STUDY ON THE CHEMOSENSITIVITY ASSAY OF HUMAN TUMOR CELL IN VITRO

作　　者：臧梦维[1] 平庆功[1] 董伟华[1] 孔天翰[1] 刘桂亭[1] 钱玉珍[1] 

机构地区：[1]附属弋矶山医院干内科,河南医科大学病理生理学教研室

出　　处：《皖南医学院学报》1995年第4期348-351,共4页Journal of Wannan Medical College

摘　　要：研究结果表明，人喉癌ＨＥ＿ｐ－２细胞接种数与克隆形成数、ＭＴＴ法ＯＤ值呈正相关、与空白对照相比，２．５×１０－４ｍｇ／ｍｌ丝裂霉素（ＭｉｔｏｍｙｃｉｎＣ，ＭＭＣ）引起克隆形成率、ＯＤ值和拒染活细胞数减少（Ｐ＜０．０１），细胞生长抑制率分别为９６．８０％、８９．５８％和７５．５１％．ＭＭＣ的抑制作用具有时间依赖关系（ｒ＝０．９９９５．Ｐ＜０．０５）。ＭＭＣ造成细胞有丝分裂指数和多核率明显低于对照（Ｐ＜０．０１）．提示ＭＭＣ对ＨＥ＿ｐ－２细胞有较强的毒性作用。本文对体外肿瘤化疗药物敏感试验的方法学进行了探讨，为进一步临床应用奠定了基础。The result indicated that the planted number of human laryngocarcinoma HEp-2 cells was closely associated with the colony foming number in the colony fomation assay and the OD value in MTT method. Compared with the blank control(BC), MMC at the concentration of 2.5×10-4mg/ ml could induce the plating efficiency,OD value and number of survival cells with dye exclusion test in HEp-2 cell to be reduced (P<0.01).Their cellular growth-inhibited rates were 96.80%,89.58% and 75.81% in above three methods,respectively. There existed a time-dependent inhibiton effect of MMC on the growth of cell (r=0.9995,P<0.05).The cell mitotic index and multineclear rate with MMC were obviously lower than those of BC (P<0.01), Which suggested that the stronger cytotoxicity on HEp-2 cell was induced by MMC. The methods of chemosensitivity assays in vitro have been inquired.The experiment foundation has been established for the further application of these assays to the clinic.

关 键 词：抗肿瘤药物 药物筛选 丝裂霉素 喉肿瘤 药敏性 

分 类 号：R979.14[医药卫生—药品]