绿色红假单胞菌和绿硫红假单胞菌的分离与鉴定  被引量:10

ISOLATION AND IDENTIFICATION OF RHODOPSEUDOMONAS VIRIDIS AND RHODOPSEUDOMONAS SULFOVIRIDIS

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作  者:杨素萍[1] 张肇铭[1] 赵春贵[2] 

机构地区:[1]山西大学生命科学系,太原030006 [2]山西大学分子科学研究所,太原030006

出  处:《微生物学报》1995年第2期91-96,共6页Acta Microbiologica Sinica

摘  要:在选择培养条件下,利用琼脂振荡稀释分离技术,对接种于造纸废水和污水处理厂污泥污水的富集培养液进行纯化,分离得到两株绿色的光合细菌菌株G和SG。它们细胞内均含细菌叫叶绿素b,光合内膜结构为片层,繁殖方式为出芽,但在对有机碳源、还原态硫化物利用能力及同化硫酸盐等生理特性上有很大差异。经鉴定,以《伯杰氏系统细菌学手册》第3卷(1989)为依据,确定这两菌株是红假单胞菌属的两个种,菌株G定名为绿色红假单胞菌(Rhndopxeudomonas viridis),菌株SG定名为绿硫红假单胞菌(Rhodopseudomonas sulfoviridis)。Under selective culture conditions, two pure cultures (strain G and strain SG) of the purple nonsulfur photosynthetic bacteria containing bacteriochlorophyll b were isolated from pulp effluent and sludge of sewage treatment plant by means of agar shake dilution. Although the cells of strain G and strain SG contained bacteriochlorophyll b and carotenoids, intra-cytoplasmic membranes were of lamellar type parallel to cytoplasmic membrane, the two strains differ in using various organic carbon sources, depending on reduced sulfur compounds and assimilate sulfate. According to Bergey's Manual of Systematic Bacteriology Volume 3, strain G was identified to be Rhodopseudomonas viridis, , strain SG was identifided to be Rhodopseudomonas sulfoviridis.

关 键 词:紫色非硫细菌 红假单胞菌属 硫化物 

分 类 号:Q939.112[生物学—微生物学]

 

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