新生隐球菌的酚氧化酶及用于菌种鉴定的研究  

THE PHENOLOXIDASE TEST AND ITS APPLICATION TO IDENTIFY CRYPTOCOCCUS NEOFORMANS WITH VARIOUS BIOLOGICAL CHARACTERISTICS

作　　者：李安生[1] 吴绍熙[1] 

机构地区：[1]中国医学科学院协和医科大学皮肤病研究所,南京210042

出　　处：《微生物学报》1995年第2期97-102,共6页Acta Microbiologica Sinica

基　　金：中国医学科学院;协和医科大学青年科学研究基金资助。

摘　　要：采用4％玉米浸汁咖啡酸琼脂(CACA)培养基。观察了具不同生物学特性的新生隐球菌的酚氧化酶活性,并对临床常见的多种酵母和酵母样真菌作了该酶的检测。结果,受试的3个变种、5种血清型和尿素酶阴性新生隐球菌均呈明确的阳性反应;150株常见酵母和酵母样真菌中43株新生隐球菌全部呈酚氧化酶阳性。107株其它酵母和酵母样真菌全部阴性。具各种不同生物学特性的新生隐球菌均特异性地产生酚氧化酶,用检测该酶的方法作该菌鉴定的特异性和敏感性均为100％,且可于72小时内得到结果。此外,结合尿素酶试验可以准确的鉴定出尿素酶阴性的新生隐球菌。Cryptococcus neoformans strains with various biological characteristics were examinated the phenoloxidase activity on the caffeic acid cornmeal agar (CACA). Other medically important yeasts were also included in this research. Firstly, thirteen reference strains of Cryptococcus neoformans were confirmly found positive phenoloxidase production on the CACA medium. Then, in total 150 isolates of yeasts, forty three Cryptococcus neoformans strains were phenoloxidase positive, other than that 107 other yeast strains were all negative. It is suggested that Cryptococcus neoformans strains with different biological features specifically and uniquely produce phenoloxidase, thus the phenoloxidase test can be applied as a useful tool to identify this pathogenic yeast. Our results also show that the culture on the CACA medium is an effective method to test the phenoloxidase activity within 72 hours. It is further approved that the urease test will not be able to be a screening test for Cryptococcus neoformans in the clinical laboratory, but it is still valuable to identify urease negative Cryptococcus neoformans when the test is used in combination with the phenoloxidase test.

关 键 词：新生隐球菌 酚氧化酶 菌种鉴定 隐球菌病 

分 类 号：R379.5[医药卫生—病原生物学] R519.404[医药卫生—基础医学]