番茄曲叶病及其血清学和PCR测定  

TRANSMISSION,SEROLOGY AND PCR ANALYSIS OF TOMATO LEAF CURL VIRUS

作　　者：蔡健和[1] 王苏燕[2] 王小凤[2] 田波[2] 

机构地区：[1]广西农业科学院植物保护研究所,南宁530007 [2]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》1995年第5期394-396,共3页Acta Microbiologica Sinica

摘　　要：我国曾报道的番茄病毒病有多种,其中最常见的是黄瓜花叶病毒(CMV)和烟草花叶病毒(TMV)引起的花叶病。柯冲等(1964)在大陆首次报道烟粉虱(Bemisia tabaci)传播的番茄病毒病——番茄黄顶病,此病在50～60年代曾在广州市郊流行,造成大面积减产。Green等(1984)报道台湾发生番茄黄曲叶病,此病与日本的番茄黄矮病(Tomato yellow dwarf)相似,并且与烟草曲叶病毒(TLCV)有血清学关系。印度、委内瑞拉等国也曾报道发生由烟粉虱传播的番茄曲叶病和番茄黄曲叶病。1991和1992年秋,在广西南宁市郊发现一种症状表现为植株矮缩,叶片向上向内卷曲,叶背面产生耳状或杯状增生物,对光看有时可见叶脉呈墨绿色,不结果或少结果的番茄病害。1992年秋广西农业科学院的番茄试验地发病率高达6.8％,对当地秋番茄生产构成了威胁。作者对病害症状、传播、血清学反应及PCR分析等方面与烟草曲叶病毒进行了比较研究,证实了该病的病原与烟草曲叶病毒有很高的同源性。现将研究结果简报如下。Tomato leaf curl virus (TomLCV) disease was found in the suburb of Nanning city of Guangxi in the autumn of 1991 - 1992. It showed the symptoms of stunting, leaf curling, cup or ear-like enation on the back of leaves, dark green vein. Test indicated that it could be transmitted by grafting and whiteflies (Bemi-sia tabaci). The infected tabacco and tomato by TomLCV induced the same leaf curl symptom as that by tobacco leaf curl virus (TLCV), based on this result, the pathogen of tomato leaf curl disease was primarily refered as TLCV. Further studies showed that both TLCV and TomLCV infected leaf extract reacted positively to MAb-SCR18 of African cassava mosaic virus (ACMV). Both virus could produce about 500bp DNA fragments by PCR using the whitefly-transmitted geminivirus common primers. Restriction enzyme analysis (using Alul, Sau3A and TaqI respectively) of these two viruses PCR products produced the same results. All the above results further confirmed that the pathogenic virus of tomato leaf curl disease has high homology with TLCV.

关 键 词：番茄曲叶病 病毒病 血清学 聚合酶链反应 

分 类 号：S436.412.1[农业科学—农业昆虫与害虫防治]