PCR法快速检测临床标本中结核杆菌DNA  

Rapid Detection of Mycobacterium Tuberculosis DNA in Clinical Samples by Polymerase Chain Reaction

作　　者：卓礼梅[1] 孙淑芹 刘芳[1] 郭辉玉[1] 

机构地区：[1]中山医科大学微生物学教研室

出　　处：《微生物学免疫学进展》1995年第1期17-21,共5页Progress In Microbiology and Immunology

摘　　要：应用聚合酶链反应（ＰＣＲ）快速检测临床标本（脑脊液、胸水、腹水、血、痰液）中的结核杆菌ＤＮＡ，特异性扩增片段１２３ｂｐ，为结核杆菌的特异性重复序列ＩＳ６１１０部分基因。ＰＣＲ检测人型结核杆菌的敏感性达１０ｆｇＤＮＡ。临床标本的ＰＣＲ检测阳性率（２３．３％）明显高于抗酸染色涂片（２．９％）和细菌培养（５．７％）的阳性率（Ｐ＜０．０５）。通过设立对照系统及对扩增产物酶切分析，表明该法无假阴性结果（特异性１００％）。研究表明，ＰＣＲ法适于快速检测不同标本中的结核杆菌ＤＮＡ。A polymerase chain reaction (PCR) assay was developed for the rapid detection of Mycobacterium tuberculosis DNA in clinical samples. The amplified product was a 123bp segment derived from the Mycobacterium tuberculosis specific insertion sequence IS6110. The assay could detect as less as 10fg of purified mycobacterial DNA, and its positive rate in testing clinical samples (23. 3% ) was significantly higher than that by conventional acid-fast stain (2. 9%) and culture techniques (5. 7%). There were no false-positive results by PCR (specificity, 100%). Our results suggested that PCR is a useful technique for the rapid diagnosis of tuberculosis at various site .

关 键 词：结核杆菌 结核病 聚合酶链反应 DNA 

分 类 号：R520.4[医药卫生—内科学]