PCR检测血清HBV　DNA  

The Detection of HBV DNA in Serum by PCR

作　　者：石乐琴[1] 王秉瑞[1] 

机构地区：[1]卫生部兰州生物制品研究所

出　　处：《微生物学免疫学进展》1995年第2期90-93,共4页Progress In Microbiology and Immunology

基　　金：卫生部青年科学研究基金

摘　　要：本文利用ＨＢＶＤＮＡ基因组ｐｒｅＣ和Ｃ区的一套引物，以ＰＣＲ法检测了２６例健康正常人血清和９５例免疫标志各异、临床症状不同的乙肝或可疑乙肝患者的血清，前者无１例检出ＨＢＶＤＮＡ，后者共检出６６例血清存在ＨＢＶＤＮＡ。该方法特异性强，适用于检测任一亚型的ＨＢＶＤＮＡ，一轮ＰＣＲ最低可检出０．１ｐｇ的ＨＢＶＤＮＡ。Studies on the detection of HBV DNA in serum by PCR using a set of primers in Pre-C and C region of HBV genome were carried out. 26 samples of normal individuals serum and 95 samples of hepatitis B or suspected hepatitis B serum with different immunological marks and clinical symptoms were tested. Among them, all 26 normal individuals were HBV DNA negative and 66 of 95 the latter were HBV DNA positive. 43 of 44 HBeAg-positive and 45 of 50 anti-HBc-positive with HBsAg presented HBV DNA, and all 4 anti-HBs-positive were negative for HBV DNA separately. In 11 samples which were negative for all immunological marks, 3 samples were HBV DNA positive. Results showed that the method we used here in diagnosing HBV DNA was superior to the routine method, both in specificity and sensitivity. It could detect 0. 1pg HBV DNA at least.

关 键 词：乙型肝炎 乙型肝炎病毒 DNA 聚合酶链反应 诊断 

分 类 号：R512.620.4[医药卫生—内科学]