狂犬病病毒Vero细胞适应株的建立及其应用于疫苗生产的可行性探讨  

Establishment of Rabies Virus strain Adapted to Vero Cell and Study on Its Possibility for Vaccine Preparation

作　　者：高鸿瑞[1] 魏至栋[1] 单秀琴[1] 阮峰[1] 赵新华[1] 袁文潭 杨辉[1] 李根明[1] 

机构地区：[1]卫生部兰州生物制品研究所

出　　处：《微生物学免疫学进展》1995年第3期129-132,共4页Progress In Microbiology and Immunology

摘　　要：将狂犬病病毒７ａＧ固定毒适应于Ｖｅｒｏ细胞，建立了稳定的ＶＲＧ适应株。病毒滴度可达７．６９ｌｏｇＬＤ５０／ｍｌ，病毒最适增殖时间５—７天，最适种毒比例１：１０￣３。应用转瓶培养Ｖｅｒｏ细胞增殖病毒，收获病毒液经β—丙内脂灭活，超离浓缩制备疫苗，效力试验结果（ＮＩＨ法）较原制疫苗成倍增高，且都大于２．５ＩＵ／２ｍｌ，表明ＶＲＧ适应株可应用于制备Ｖｅｒｏ细胞狂犬病疫苗。Rabies virus strain 7aG was adapted to Vero cell by cellmixing culture, subculture and cell－free medium culture, designated as VRG strain. The virus were serially passaged on Vero cell for up to 19 passages,the viral titers of supernatant of 17th, 18th and 19th passage reached 7. 63, 7. 69 and 7. 52 logLD50/ml ,respectively, i. e these titers were maintaining the same level.The optimal time of virus propagation was 5 to 7days,the optimal virus inoculative ratio was 1 :103. The results showed that the 7aG rabies virus strain was adapted to Vero cell completely. Potency of concentrated vaccine by ultracentrifugation is higher than 2. SIU/2ml (by NIH method).It is two times higher than that of crude vaccine. The VRG strain is capable to be applied to Vero cell preparation of rabies vaccine.

关 键 词：狂犬疫苗 狂犬病 疫苗 VERO细胞适应株 制备 

分 类 号：R512.990.3[医药卫生—内科学] R392-33[医药卫生—临床医学]