尿2－硫代噻唑烷－4－羧酸的反相高效液相色谱法研究  

Determination of urinary 2-thio-thiazolidine-4-carboxylic acid by reversed-phase high performance liquid chromatography

作　　者：简乐[1] 

机构地区：[1]浙江医科大学预防医学系

出　　处：《卫生研究》1995年第5期257-259,共3页Journal of Hygiene Research

基　　金：浙江省卫生厅青年科学基金

摘　　要：应用反相高效液相色谱法（分离柱：ＵｔｒａｓｐｈｅｒｅＴＭＯＤＳＣ－１８）对二硫化碳（ＣＳ２）代谢产物２－硫代噻唑烷－４－羧酸（ＴＴＣＡ）进行了测定条件优选。以流动相为甲醇（１４％）、冰乙酸（１. ５％）和水（８４. ５％）；流速１. ５ｍｌ／ｍｉｎ；紫外线检测波长２７２ｎｍ时分离检测效果最好。同时，对尿样前处理方法也进行了探讨。本法平均回收率９８. ９％，最小检出量０. ００８ｎｇ，ＴＴＣＡ浓度在０～２００ｍｇ／Ｌ范围内呈直线相关（ｒ＝０. ９９９６）是ＣＳ２生物监测的良好方法。A method using reversed-phase high performance liquid chromatography with Ultrasphere ODS C-18 column was performed on the urinary 2-thiothiazolidine-4-carboxylic acid (TTCA), one of the metabolites of CS2. It was found that the separating efficiency was high when the mobile phase was composed of methanol (14%), glacial acetic acid (1.5%), and water (84.5%).The detector wavelength was adjusted to 272 nm and flow rate was set at 1.5ml/min.The calibration curve was linear over the range of 0～200 mg/L.The average recovery ratio was 98. 9% and the detection limit was 0.008ng.The method is sensitive, accurate and useful for biological monitoring of CS2 exposure.

关 键 词：二硫化碳 生物监测 尿 代谢产物 TTCA 液相色谱 

分 类 号：R135.1[医药卫生—劳动卫生]