草甘膦对大鼠肝微粒体酶活力的影响及其机理初探  

The effects of glyphosate on liver microsomal enzymes and possible mechanisms in rats

作　　者：严红[1] 邬惠琼[1] 柴莲花[1] 王新刚[1] 

机构地区：[1]同济医科大学环境毒理教研室

出　　处：《卫生毒理学杂志》1995年第4期213-216,共4页Journal of Health Toxicology

摘　　要：运用体内试验和体外试验的方法，研究了草甘膦对大鼠肝微粒体酶活力的影响，并对其作用机理进行了初步探讨。结果表明，体内试验中，草甘膦能明显增加细胞色素Ｐ４５０含量，诱导氨基比林Ｎ－脱甲基酶（ＡＰＮＤ）和乙基吗啡Ｎ－脱甲基酶（ＥＭＮＤ）的活力；体外试验中，在３．３×１０－８～３．３×１０－４ｍｏｌ／Ｌ浓度范围内，未见草甘膦对Ｐ４５０含量有明显增加，而在２×１０－６ｍｏｌ／Ｌ和２．５×１０－５～２×１０－４ｍｏｌ／Ｌ浓度范围内可分别对ＡＰＮＤ和ＥＭＮＤ呈现诱导作用，在２×１０－８～２×１０－４ｍｏｌ／Ｌ浓度范围内，随草甘膦浓度增高，ＡＰＮＤ和ＥＭＮＤ活力均有上升趋势。初步观察结果，草甘膦在体内对肝微粒体酶诱导作用机理与其促进蛋白质合成作用有关。The present study was undertaken to investigate the influence of Glyphosate on the activities of liver microsomal enzymes in rats.A preliminar study was also made to elucidate the possible mechanisms of these effects.Following conclusions were drawn:In vivo,microsomal cytochrome P450 content and activities of APND.EMND were significantly increased by Glyphosate;In vitro,no obvious change was observed in content of cytochrome p450 by Glyphosate at 3.3×10-6￣3.3×1-4mol/L.While at 2X10-6￣2×10-4mol/L and 2.510-5￣2×10-4mol/L,APND and EMND could be significantly induced by Glyphosate respectively.The activities of APND and EMND were enhanced with the increase of Glyphosate concerntration at 2X10-8￣210-4mol/L.The inducing of Glyphosate on liver micerosomal enzymes was possibly related to its promoting action in enzyme protein synthesis in body.

关 键 词：除草剂 微粒体酶 细胞色素P450 酶动力学 草甘膦 

分 类 号：TQ450.261[化学工程—农药化工]