硝酸铅对CHL有丝分裂细胞纺锤体的损伤作用  

Mitoic spindle damage induced by iead nitrate in CHL cells

作　　者：高沛永[1] 王琼[1] 

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《卫生毒理学杂志》1995年第4期228-230,共3页Journal of Health Toxicology

基　　金：国家自然科学基金

摘　　要：用不同浓度的硝酸铅（０．０６～９．６０ｍｍｏｌ处理ＣＨＬ细胞２４小时后，有丝分裂纺锤体和染色体分化染色的实验结果表明，随着硝酸铅浓度的升高，各种类型的纺锤体异常都有剂量依赖性升高，其中非中板集合、多极、Ｃ－中期的最高值（％）分别达到２１．８，２３．０，１４．７．在本实验的最高浓度，已观察不到纺锤体形态正常的细胞，球形中期占１００％。我们就铅对有丝分裂周期的阻滞作用及纺锤体毒性的特点。After treatment of CHL cells with different concentrations of lead nitrate for 21 hours, the experimental results of differential staining of mitoic spindle and chromosomes show that following the increase of lead nitrate concentration the percentages of several kinds of spindle disturbance increase in dose-dependent manner and the maximal value of non-congression,multipolar,c-metaphase reaches 21.8,23.0 and 14. 7% respectively.At the maximal concentration of lead nitrate in this experiment there is no cell with normal spindle morphology can be seen and the ball metaphase reaches a hundred percent.The block effect of lead on the mitotic cycle and some traits of the spindle toxicity of lead were discussed in the paper.

关 键 词：硝酸铅 CHL细胞 有丝分裂周期 纺锤体 毒性 

分 类 号：R995[医药卫生—毒理学]