九连小檗悬浮培养细胞生理生化特性的研究　Ⅴ．细胞生长与呼吸作用及对主要营养元素吸收、消耗的关系  

STUDIES ON PHYSIOLOGICAL AND BIOCHEMICAL CHARACTER IN SUSPENSION CULTURE OF BERBEBIS JULIANAE SCHNEID CELLS Ⅴ.CELL GROWTH,RESPIRATION,ABSORPTION AND CONSUMPTION OF MAJOR NUTRITIONAL ELEMENTS

作　　者：侯嵩生[1] 李新明[1] 李洪林[1] 吴玉兰[1] 

机构地区：[1]中国科学院武汉植物研究所

出　　处：《武汉植物学研究》1995年第2期163-166,共4页Journal of Wuhan Botanical Research

摘　　要：叙述了九连小檗细胞悬浮培养中细胞生长与呼吸作用及对主要营养元素吸收消耗的关系。细胞接种于新鲜培养液中，呼吸作用强度和对主要营养元素的吸收、消耗便迅速增加。接种后第６天，培养液中ＰＯ４３－几乎被耗尽，Ｋ＋和ＮＯ３－的浓度也减少一半，细胞生长由延迟期进入起始生长期。至第８天呼吸强度达到高峰，细胞生长加速，但细胞生长高峰延迟于呼吸高峰。随后，营养物质的消耗减少，呼吸降低，生长减慢。至静止期细胞生长停止，呼吸作用微弱。这些结果为优化培养条件，改进培养基组成，调控细胞生长和代谢提供了参考。This paper reports the relationship between cell growth,respiration,and absorption, consumption of major nutritional elements in suspension culture of Berberis julianae Schneid cells.During the cells were transferred into fresh medium,cell respirationintension and absorption of major nutritional elements were increased rapidly.Respiration intension was maximal about on 8th day after inoculation.Phosphate ion(PO43-)was completely depleted and half of K+ and NO3- were consumed in the medium on 6th day after inoculation. The change of respiration intension is related to the absorption of major nutritional elements in the medium.The absorption rate of (PO43-)is faster than that of K+and NO3-.Cells continued to grow well after (PO43-)had disapeared from the medium.This results are benifited the optimize medium composition for biomass production.

关 键 词：九连小檗 细胞生长 悬浮培养 生理生化特性 

分 类 号：Q949.746.8[生物学—植物学]