荧光探针法研究囊泡融合的动力学  

Fluorescence Method for Studying the Fusion of Vesicle

作　　者：何煦[1] 顾新华[2] 赵国玺[1] 戴光松[2] 吴世康[2] 

机构地区：[1]北京大学化学系,北京100871 [2]中国科学院感光化学研究所,北京100101

出　　处：《物理化学学报》1995年第6期504-508,共5页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金

摘　　要：应用荧光探针法研究双（月桂酸）三乙醇胺酯形成囊泡的融合动力学过程，工作中合成和应用了含长烷基链的荧光探针—十六烷基罗丹明B，以保证其进入囊泡的双分子层，随着融合的进行，探针浓度被稀释，探针分子逐渐由二聚体离解成为单体，从而使其荧光强度增强．研究发现，由双（月桂酸）三乙醇胺酯形成的囊泡，其融合速率和膜结构与介质酸度、环境温度等密切相关，当介质酸度处于一定的pH范围时，形成的囊泡膜具有最致密的结构，其融合也最慢。Fluorescence method was used to measure the kinetics of fusion of vesicles,which was formed by triethanolamine dilaurate by means of ultrasoaic dispersion. The method relies upon the dequenching of Cetyl Rhodamine B (CERB) in vesicles. The concentration of CERB was diluted with the fusion, and dimer of CERB was dissociated into the monomer, resulting in the enhancement of fluorescence intensity of CERB. Result indicated that the fusion rate of the vesicles formed by triethanolamine dilaurate closely related to the structure of bilayer and acidity of the media, and when the acidity of the media was in the certain range of pH, the vesicles formed had closer packed structure as well as slower fusion rate.

关 键 词：荧光探针 囊泡融合 CERB 

分 类 号：Q73[生物学—分子生物学]