诸葛菜花粉的低温保存研究  

LOW TEMPERATURE POLLEN PRESERVATION Of Orychophragmus violaceus

作　　者：赵云 王茂林 王天叫 

机构地区：[1]四川联系大学生物工程系

出　　处：《西南农业学报》1995年第3期65-69,共5页Southwest China Journal of Agricultural Sciences

摘　　要：室温下，诸葛菜（Ｏｒｙｃｈｏｐｈｒａｇｍｕｓｖｉｏｌａｃｅｕｓ）花粉寿命较短，７天后全部失去生活力，在４℃低温下，花粉寿命延长至１５天，未干燥的新鲜花粉不耐冰冻，干燥花粉在－２０℃保存４０天时，萌发率降至２５％，在液氮（ＬＮ）中，干燥花粉生活力基本不变，经ＤＭＳＯ溶液预处理的冰冻花粉生活力很低，在－２０℃，花粉游离脯氨酸（Ｐｒｏ）含量随储存时间延长微弱下降．在ＬＮ中，花粉游离Ｐｒｏ含量不随时间变化。与新鲜花粉相比，ＬＮ保存花粉的游离Ｐｒｏ含量略有升高，但不显著，冰冻（－２０℃，ＬＮ）保存２０天的花粉均能正常授粉结实。Pollen preservation of O.violaceus was studied preliminarily.The result showed that Pollen life span wus shorter al room temperatute.Pollen viability was fort completely in 7 days at room remnperature or in 15 days at 4℃.Non dried Pollens could not risist to frost. During a period of 40 days.the survival Percentage of dried pollens stored at-20℃ declined to 25%.whereas the viability of dried pollens stored in liquid nitrogen(LN) remained constant.The viabillty of frozen pollens ptetrealed with DMSO solution was very low.As time went out,the content of free Pro in pollens declined slightly at-20℃ and did not change in LN.Compared with fresh Pollens.the content of free Pro in Pollens stored in LN rose up a little,but the difference was not remarkable. Pollens.stored respectively at-20℃and in LN for 20 days.could be used to pollinate and the pollinated flowers developed fruit and seeds.

关 键 词：诸葛菜 花粉保存 种质 油料作物 

分 类 号：S565.902.4[农业科学—作物学]