PCR和ELISA对单纯疱疹病毒Ⅰ型脑炎的诊断价值  

The Diagnosis Significance of PCR and ABC-ELISA in Herpes Simplex Virus En-cephalitis

作　　者：王宁山[1] 侯熙德[1] 金庆文[1] 姚娟[1] 

机构地区：[1]南京医科大学一附院神经科

出　　处：《现代诊断与治疗》1995年第3期144-145,共2页Modern Diagnosis and Treatment

摘　　要：应用ＰＣＲ和ＡＢＣ－ＥＣＩＳＡ法同步检测１０４例临床拟诊病毒性脑炎患者配对脑脊液和血清１３６份。其中２１例患者的３４份配对标本特异性ＩｇＧ阳性及３１例患者的４１份脑脊液中ＨＳＶ－Ⅰ型ＤＮＡ阳性，判为单纯疱疹病毒性脑炎。ＰＣＲ在病早期阳性率较高，病中晚期ＩｇＧ阳性较集中，二法可在临床诊断中互补．In this study, 136 paired serum andpaired CSF samples from 104 patients with sporadicviral encephalitis have been examinated for diagnosisof HSV-specific DNA and antibody by PCR andABC-ELISA.The result of PCR and ABC-ELISAhave been compared and contrast to develop generaldirection for virological diagnosis of HSVE. Of 41CSF from the 31 patients,the HSV-specific DNAwere positive and of 34 paired serum and CSF from21 patients,the specific antibody were positive,In36 samples from 26 patients with non-HSVE centralnervous system disorders,specific DNA and specificantibody were negative by two methods.The studysuggested that these two methods should be intro-duces in routine diagnosis of viral encephalitis.

关 键 词：疱疹病毒 脑炎 脑脊液 聚合酶链反应 ELISA 诊断 

分 类 号：R512.304[医药卫生—内科学]