应激对大鼠行为和部分脑区谷氨酸含量的影响  

THE EFFECT OF STRESS ON BEHAVIORS AND GLUTAMATE LEVELS OF FOUR BRAIN REGIONS

作　　者：严进[1] 王春安[1] 叶阿莉[1] 陈宜张[1] 

机构地区：[1]第二军医大学生理学教研室神经科学实验室

出　　处：《心理学报》1995年第4期422-427,共6页Acta Psychologica Sinica

基　　金：全军医药卫生科研基金

摘　　要：以旷场试验法测定动物在急、慢性躯体性和心理性应激时的行为变化，以快速断头冷冻匀浆法，用ＨＩＴＡＣＨＩ－８３５氨基酸分析仪测定应激各期视皮层、海马、下丘脑、小脑谷氨酸含量。并对正常动物经侧脑室微量注射Ｌ－ＡＰ４后观察行为变化。结果显示，急性应激期动物行为活动增加，慢性应激期减少：应激时，大鼠部分脑区的谷氨酸含量与对照组相比在不同时期呈显著性差异；侧脑室微量注射Ｌ－ＡＰ４提示行为活动减弱可能与脑内Ｇｌｕ系统的活动有关。Glutamate(Glu)levels of the visual cortex(VC) ,hippocampus (Hi),hypothalamus(Hy)and cerebellum(Ce)during acute and chronic,and physical and psychological stress in rats were measured.Open-field behaviors (OFBs)during stress and after intracerebroventricularly(icv,lateral ventricle) administered L-AP4(10-3M/L,5ul)or saline(5ul)were recorded with TV camera The results showed that Glu levels of Hi and Hy in acute stress group and that of VC in chronic stress groups were higher and that of Ce was lower than that of the control (P<0.05),and there was a significant difference in Glu level of Hi between physical and psychological stress.OFBs were markedly reduced during chronic stress.It was similar to chronic stressed animals that OFBs in non-stress rats were reduced significantly in the 5min following icv administration of L-AP4.These suggested that stressinduced OFBs change was connected with Glu system in CNS.

关 键 词：应激 旷场试验 谷氨酸 大鼠行为 

分 类 号：B845[哲学宗教—心理学]