肾炎肾组织中HBVDNA的检测及其存在意义  

THE EXISTENCE OF HBV DNA IN THE RENAL TISSUE OF GLOMERULONEPHRITIS AND ITS SIGNIFICANCE

作　　者：周士东[1] 张月娥[1] 方利君[1] 蒋惠秋[1] 王万胜[1] 顾建人[1] 

机构地区：[1]上海医科大学病理学教研室,上海医科大学附属儿科医院,上海市肿瘤研究所

出　　处：《徐州医学院学报》1995年第4期360-363,共4页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金

摘　　要：应用Ｓｏｕｔｈｅｒｎ印迹分子杂交和免疫组化技术，检测１８例肾穿刺组织中ＨＢＶＤＮＡ和ＨＢＶ相关抗原（ＨＢｓＡｇ、ＨＢｃＡｇ和ＨＢｅＡｇ）的阳性情况。结果表明１５例ＨＢＶＤＮＡ阳性，其中８例成人和４例儿童为整合型，另３例儿童为游离型；有１３例ＨＢＶ抗原阳性。除肾小球外，肾小管亦麦达ＨＢＶ抗原，提示ＨＢＶＤＮＡ的存在状态在成人主要为整合型，在儿童有整合型和游离型。讨论了ＨＢＶＤＮＡ的存在与ＨＢＶ抗原表达的关系。HBV DNA in renal biopsy specimens from 18 glomerulonephritis patients were detected by southern blot hybridization,with the HBV antigens in them examined simultaneously by immunohistochemical techniques.It was found that HBV DNA in the renal tissue were positive in 15 cases and appeared in 2 forms,i.e.integrated HBV DNA in 8 adults and 4 children and non-replicative free HBV DNA in the other 3 children.HBV antigens were found in 13 of the 15 HBV DNA positive renal tissue specimens.It can be seen that the HBV DNA in renal tissue was mainly of integrated form in adults and was of either free or integrated form in children. The relationships between the existence of HBV DNA in renal tissue and the expression of HBV markers in infected renal tissue and blood serum are discussed. The significance of HBV DNA and HBV markers in renal tissue in the diagnosis of HBV-related nephritis is defined.

关 键 词：肾炎 乙型肝炎病毒 免疫组织化学 脱氧核糖核酸 

分 类 号：R692.304[医药卫生—泌尿科学] R446.5[医药卫生—外科学]