应用DNA指纹图谱法对湘白猪群体遗传结构的研究  

STUDY ON GENETIC STRUCTURES OF HUNAN WHITEPIG POPULATIONS BY DNA FINGERPBINTING

作　　者：孙宗炎[1] 唐煌辉 杨仕柳[1] 孟安明[1] 

机构地区：[1]湖南省畜牧兽医研究所,北京农业大学生物学院

出　　处：《畜牧兽医学报》1995年第1期29-34,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：本文采用Ｊｅｆｆｒｅｙｓ小卫星探针３３．６和３３．１５检测了４个湘白猪品系（Ⅰ、Ⅱ、Ⅲ、Ⅳ）和５个亲本品种（大约克、长白、杜洛克、大围子、沙子岭）的ＤＮＡ指纹图谱。根据指纹带型计算了每个群体的相似系数（Ｆ）、平均等位基因频率（ｑ）和最低平均杂合率（Ｈ）。４个湘白猪品系的分析结果表明：湘白猪各品系内的相似系数为０．４５～０．４９，接近于欧美引进品种长白猪（０．４８～０．５０），具有较好的遗传纯合性。根据９个品种（系）的群体ＤＮＡ指纹图谱进行的聚类分析结果表明：在９个品种系的遗传背景下４个湘白猪品系最先聚为一类，从而在分子水平上证实了４个品系具有了构成同一个品种（湘白猪）所需的“遗传相似关系”。聚类图所反映的遗传相似关系与品种培育历史一致。Two human minisatellite probes 33.6 and 33.15 were used to determine the Patternsof DNA fingerprinting (DFP) in four Hunan White pig (HW) strains and their fiveparental breeds (Yorkshire, Landrace, Duroc, Daweizi and Shazilin). Similarity coeffici-ents(F), mean allelic frequencies(q) and minimun heterozygosities(H) were calculatedbased on DFP. Analysis of DFP in four HWP strains indicated that F within each HWstrain ranged from 0.45￣0.49 which ivas close to the F (0.48￣0.50) for the Landr-ace breed. Therefore, it can be concluded that each HW strain has reached a higherdegree of homogeneity. A parsimony analysis made on the basis of DFP patterns in allnine pigs populations was given, four HW strains clustered first. This result confirmedthat all four HW strains had already possessed the common genetic basis needed forconstructing an identical breed. The groupings illustrated by the parsimony trees were.consistent with the historical records of HW breeding.

关 键 词：猪 DNA 指纹图 小卫星探针 遗传相似性 

分 类 号：S828.2[农业科学—畜牧学]