猪瘟兔化弱毒犊牛睾丸细胞苗免疫程序研究  

STUDIES ON THE IMMUNE-PROCEDURE OF LAPINIZEDATTENUATED SWINE FEVER VACCINE PREPARED INCALF TESTlCLE CELLS

作　　者：郑自才 石谦[1] 黄昌祥[1] 江潮元 

机构地区：[1]四川省内江市东兴区畜牧中心,四川省畜牧局,四川省德阳市中区畜牧局

出　　处：《畜牧兽医学报》1995年第2期160-167,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：猪瘟（ＨＣ）犊睾细胞苗７５０个免感染量，免疫空怀母猪所产亲代仔猪取血清监测其母源传递抗体ＥＬＩＳＡ效价。１０日龄ＯＤ值＞０．３０６３％（１９／３０），０．３０≥ＯＤ值＞０，２０３７％（１１／３０），保护率为９０．８５％；２０日龄ＯＤ值＞０．３０２８％（８／２９），０．３０≥ＯＤ值＞０．２０　５５％（１６／２９），保护率为６８．９６％；３０日龄ＯＤ值＞０．３０７％（２／２９），０．３０≥ＯＤ值）０．ｍ４１％（１２／２９），保护率为３７．９３％；４０日龄ＯＤ值＞０．３０００．３０≥ＯＤ值＞０．２０１７％（５／２９），保护率为１２．９％。表明仔猪母源抗体消失极快，３０日龄母抗已有６０％以上不能抵抗ＨＣＶ侵袭。仔猪３０日龄免疫ＨＣ犊宰细胞苗１５００个兔感染量，待免疫９个月（２７４±３ｄ），１０个月（３１１±３６）取血清监测其免抗ＥＬＩＳＡ效价为ＯＤ值＞０．３０１００％（２５／２５），保护率１００％和ＯＤ值＞０．３０７６％（１９／２５），０．３０≥ＯＤ值＞０．２０２４％（６／２５），保护率９４％。试验证明了仔猪３０日龄免疫１５００个兔感染量每头猪免疫后到出栏（１０个月）具有坚强的保护性抗体。东兴区１９９２年仔猪阉割免?By inoculating non-pregnant sows with 750 lapin infective doses of Swine FeverVaccine Prepared in Calf Testicle Cells Per head and monitoring the titers of thematernal antibody in the baby pigs of Parental generation with ELISA method, we havegotten the following results: For 63%(19/30) of the 10-dayold pigs, the OD valuewas>0.3 and for 370%(11/30) of them, the OD value was 0.3 to >0.2. The rate ofprotection was 90.850%. For 20-day-old pigs, for 28% of them (8/29), the OD valuewas >0.3 and for 55% (16/29) of them, the OD value was %0.3 to>0.2. The rateof protection was 68.960/0. For 30-day-old pigs, there were only 7% (2/29) of themwhose OD value was >0.3, those whose OD value was 0.3 to>0.2 made up 41%(12/29). The rate of protection was 37.93%. For 40-day-old pigs, there was nonewhose OD value was >0.3. Those whose OD value wasg 0.3 to >0.2 made up 17%(5/29). The rate of protection was 12.9% The results sliow that the maternal antibodydisappears very fast. Over 60% of the pigs of 30 days old could not resist the challengeof HCV any more.Inoculating pigs of 30 days old with 1500 lapin infective doses of Swine FeverVaccine Prepared in Calf Testicle Cells per head, we got tlie titers of inimunity andresistance by using the ELISA method in serum taken from the pigs 9 months (274days 13 days) or 10 months (311 days 13 days) post inoculation. The results were asthe following: For 100% of the 9-month-old Pigs (25/25), the OD value was >0.3.The rate of protection was 100%. For 76% (19/25) of the pigs of ten months old,the OD value was >0.3 and for 200% of them (6/25), the OD value was 0.3 to>0.2. The rate of protection was 94%The experiments have shown that by inoculating pigs of 30 days old with 1500lapin infective doses per head, we could keep the pigs, protecive antibody strong until10 months later. We castrated and inoculated about 570000 pigs of 30 days old (andsome non-pregnant sows as well) in Dongxing county in 1992. All the inoculated pigsdid not have HC or non-typical HC for the whole year ronnd. Eiglit znonth

关 键 词：兔感染量 非典型猪瘟 免疫程序 猪瘟犊睾细胞苗 

分 类 号：S859.797[农业科学—临床兽医学] S852.4[农业科学—兽医学]