抗鼠隐孢子虫单克隆抗体的研究  被引量:5

STUDIES ON MONOCLONAL ANTIBODIES AGALNST OOCYSTS OF CRYPTOSPORDIUM MURIS

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作  者:胡景辉[1] 蒋金书[1] 贺争鸣[1] 

机构地区:[1]北京农业大学兽医学院

出  处:《畜牧兽医学报》1995年第5期420-425,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘  要:利用鼠隐孢子虫(Cryptospridiummuris)卵囊腮囊物免疫BALB/C小鼠,取脾细胞与SP2/O细胞融合,经过3~4次有限稀释法克隆化,获得5株持续分泌抗体的杂交瘤细胞株。所获抗体均属IgG_1。IFA检测结果表明:2G_7株单抗作用于卵囊壁抗原,而1M_4、2E_7、3G_3、4A_7株单抗作用于子孢子表面抗原。诱生腹水经过反复冻融、冻干及硫酸铵沉淀后,抗体活性无明显变化。5株单抗均不与贝氏隐孢子虫(C.baileyi)及柔嫩艾美尔球虫(Eimeriatenella)产生交叉反应,与小隐孢子虫(C.paruum)则有不同程度的交叉反应。Five hybridomas stably producing monoclonal antibodies against oocyst antigens of Cryptosporidium muris have been derived from fusion of the spleen cells of immunized BALB/C mice and SP_2/0 cells by 3-4times of limiting dilution cloning.Immnoglobulinsubclass of 5 iines were determined to be IgG1.Detected by IFA, McAb (2G_7)wasagainst epitopes on the walls of oocysts,McAbs(1A_4,2E_7, 3G_3, 4A_7)were againstepitopes on the surface of sporozoites.The reactivities of McAbs were not affectedsignificantly by freeze-drying,repeatly freezing-thawing or precipitating with ammoniumsulphate.Five McAbs did not react at all with C.baileyi and.tenella,but gave rea-ction with C.Paruum in different intensities.

关 键 词:单克隆抗体 小鼠 隐孢子虫病 人兽共患病 

分 类 号:S855.912.3[农业科学—临床兽医学]

 

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