牛体外成熟卵母细胞冷冻保存的研究  

SIUDY ON IHE CBYOPRESERVAIION OF IN VITRO MATURED BOVINE OOCYTES

作　　者：冯怀亮[1] 孙青原[1] 李子义[1] 杨庆章[1] 刘国艺[1] 秦鹏春[1] 

机构地区：[1]东北农业大学生物工程系,中国人民解放军农牧大学基础部,中国科学院动物所生殖生物学国家重点实验室

出　　处：《畜牧兽医学报》1995年第6期481-486,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：黑龙江省自然科学基金;吉林省科委基金

摘　　要：本实验探讨了用６种不同方法冷冻保存的牛体外成熟卵母细胞体外受精后的发育潜力及其冷冻损伤。尽管用不同方法冷冻的牛卵母细胞多数（８６．１％）形态正常，仅体外受精后的受精率（３０．０％）和卵裂率（７．４％）明显低于对照组（６９．０％和５２．９％），卵裂后继续发育的能力严重受损。在６种冷冻方法中，１．６Ｍ丙二醇分步平衡、程序冷冻效果最好，受精率和卵裂率分别达３６．４％和１３．５％。冷冻后卵母细胞发育潜力下降的原因是皮质颗粒胞吐引起的透明带变硬及亚细胞结构损伤。玻璃化法冷冻的卵母细胞损伤较轻，是值得进一步研究的一种方法。The developmental competence and cryoinjuries of in vitro matured(IVM)bovineoocytes cryopreserved by 6 different methods were studied.Altliough most of the IVMoocytes(86.1%)appeared morphologically normal after freezing and thawing,thefertilization rate(30.0%)and cleavage rate(7.4%)were significantly lower than thoseof control group(69.0%and 52.9%)after in vitro insemination and the developmentalcompetence of 2-cell embryos derived from cryopreserved oocytes was also severelydamaged.Among the 6 cryopreservation methods,step equilibration with 1.6M PG andprogrammal cooling achieved the highest fertilization rate(36.4%)and cleavage rate(13.5%).The reasons for the decreased developmental competence were zona hardeningcaused by cortical granule exocytosis and the destruction of subcellular structure;Theinjuries of vitrified oocytes were less severe and this method was worthy of furtherstudy.

关 键 词：牛 卵母细胞 冷冻保存 

分 类 号：S823.34[农业科学—畜牧学]