抗虫转基因烟草8611纯合系的选育  

Selection of Transgenic Tohacco Cultivar 8611 Highly Resistant to Insects

作　　者：吴中心[1] 张同庆[1] 姚根怀 朱祯[1] 刘春明[1] 李向辉[1] 

机构地区：[1]河南省农科院烟草研究所,中国科学院遗传研究所

出　　处：《烟草科技》1995年第1期21-24,共4页Tobacco Science & Technology

摘　　要：对获得的表达江豆胰蛋白酶抑制剂的转基因８６１１进行了抗虫性研究，从转基因当代植株中筛选出两株高抗单株８６１１－６、８６１１－１１，在用其离体叶片饲养烟方虫８天后，对烟青虫的杀死率分别达到５０％和４０％。在田间接种及自然（不施药）条件下，８６１１－６、８６１１－１１子代植株的虫害指数分别为６３．５％、６９．３％（接种后１５天）和３７．７％、３９．８％（移栽后２５天），而未转基因对照植株的虫害指数分别为９３．５％和５７％。The resistance to insects of the transf1enic tobacco cv 8611 expressing CPTI gene was tested. In comparitson with the control,mortality of the insects feedL1d on transgenic plants 8611.6 and 8611-11 reached 50%and 40%respectively,and the growth the:urvived insects were remarkeably inhibited.Under field condition,the in sects-damage index of progenies of 8611-6 and 8611-11 averaged 63.5%and 69.3/15 days after inoculation,averaf1ed 37.7%and 39.8%25 days after transplant,while that of the non-transformed control were 93.7%and 57%。 these results demonstrated that it was possible to obtain insect protection in transgenic tobacco expressing CPTI gene。

关 键 词：转基因 8611纯合系 抗虫性 烟草 选育 

分 类 号：S572.034[农业科学—烟草工业]