干扰素-γ、干扰素-α对体外培养的人视网膜色素上皮细胞的影响  

Inhibition effects of interferon-gamma and interferon-alpha on human retinal pigment epithelial cells in vitro

作　　者：王兆艳[1] 惠延年[1] 胡明[1] 

机构地区：[1]第四军医大学西京医院眼科,710032

出　　处：《眼科》1995年第3期173-175,共3页Ophthalmology in China

摘　　要：目的:探讨干扰素-γ、干扰素-α能否抑制玻璃体内细胞增生。方法:应用~3H-胸腺嘧啶核苷(~3H-TdR)掺入及液体闪烁测量技术,观察不同浓度的干扰素-γ、干扰素-α(10IU/ml～10~4IU/ml)在3天、5天两个时间点对体外培养的人视网膜色素上皮细胞的影响。结果:在3天时观察,干扰素-γ对人视网膜色素上皮细胞有轻度的抑制作用,最大(10~4IU/ml)抑制率为26%(P<0.05),干扰素-α对视网膜色素上皮细胞有轻度的抑制作用,最大(10~4IU/ml)抑制率为26%(P<0.05)干扰素-α对视网膜色素上皮细胞的抑制作用,较干扰素-γ弱(10~4IU/ml时抑制率为16%)。5天时观察,抑制作用较3天时减弱。结论:干扰素-γ、干扰素-α对人视网膜色素上皮细胞有轻度的抑制作用,其作用维持3天左右。PURPOSE. To evaluate the effects of interfe-ron-gamma (IFN-γ) and interferon-alpha (IFN-α) on human retinal pigment epithelial (RPE) ceils in vitro. METHODS: Cultures of human RPE cells were incubated with either of the IFNs (10IU/ml～10~4 IU/ml) for 3～5 days, and proliferation rates of the cells were assayed by [~3H]-thymidine incorporation and liquid scintillation techniques. RESULTS: There was a slight inhibition on human RPE cells with dosages from 10IU/ml to 10~4IU/ml. The inhibition rates of IFN-γ of 10~3IU/ml, 10~4IU/ml were 8%, 15%, 26%, and of IFN-α were 7%, 9%, and 16%, respectively. On the 5th day there was a slight decrease in the inhibition rate than on the 3rd day. CONCLUSION: IFN-γ and IFN-α exerted a slight inhibition on human RPE cells lasting 3 days.

关 键 词：干扰素类 视网膜色素上皮 上皮细胞 药理学 

分 类 号：R978.7[医药卫生—药品]