钙离子对4－氨基吡啶诱发肥大细胞释放组胺作用的影响  

INFLUENCE OF CALCIUM CONCENTRATION ON 4-AMINOPYRIDINE INDUCED HISTAMINE RELEASE FROM ISOLATED PEKITONEAL MAST CELLS

作　　者：徐建华[1] 王佩[1] 陈志刚[1] 张剑 

机构地区：[1]浙江医科大学药学系药理学教研室

出　　处：《药学学报》1995年第3期235-238,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：钙离子对４－氨基吡啶诱发肥大细胞释放组胺作用的影响徐建华，王佩，陈志刚，张剑　（浙江医科大学药学系药理学教研室杭州３１０００６）４－氨基吡啶（４－ａｍｉｎｏｐｙｒｉｄｉｎｅ，４－ＡＰ）是一种钾通道阻滞剂，它能促进神经末梢释放多种递质［１，２］，促进β...Aminopyridine(4-AP) has been shown to induce histamine release fromisolated peritoneal mast cells(PMC)in mice and rats. In the presence of extracellular calcium atnormal level(0.9 mmol·L-1)histamine release induced by 4-AP(13. 6 mmol·L-1) from mice PMCwas 33.0%±4.6%,while at low calcium concentration (0.5 mmol·L-1) and in calcium-freemedium this parameter decreased to 25.5%±4.2%and 16.3%±3.7%respectively.Histaminerelease in response to 4-AP(10 mmol·L-1)from rat PMC was 39.1%±6.7%(0.9 mmol·L-1calcium),while at low calcium concentration(0. 5 mmol·L-1) and in calcium-free medium thisparameter decreased to 29.3%±4.7%and 20.2%±2.9% respectively. Results of statisticalanalysis indicate that 4-AP induced histamine release is related to Ca2+ concentration. When rat PMCwere preincubated in calcium-free medium with EDTA 0.1 mmol·L-1 for 180 min 4-AP inducedhistamine release was 13.8%±1.6%. This shows that 4-AP also elicited mobilization of endogenous calcium stores in mast cells.The mechanism of 4-AP induced histamine release was discussed.

关 键 词：钙离子 4-氨基吡啶 肥大细胞 细胞释放 药理 

分 类 号：R962[医药卫生—药理学]