反相高效液相色谱法测定人血浆中双氯灭痛  

Determination of Diclofenac Sodium in Human Plasma by RP-HPLC

作　　者：李克[1] 赵飞浪[1] 袁倚盛[1] 谭力[1] 

机构地区：[1]南京军区南京总医院中心仪器分析科

出　　处：《药物分析杂志》1995年第5期17-19,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文建立了用反相高效液相色谱法测定人血浆中双氯灭痛的方法，对样品提取及色谱分析条件等作了详细研究。血浆经酸化后用正己烷－异丙醇（９５：５，Ｖ／Ｖ）提取，以ＳｐｈｅｒｉｓｏｒｂＣ＿（１８）为固定相，甲醇与醋酸钠缓冲液的混合溶液（６８：３２，Ｖ／Ｖ；ｐＨ４．２）为流动相进行色谱分离。检测波长为２７４ｎｍ，二苯胺为内标。双氯灭痛浓度在０．１０～２．５０μｇ／ｍｌ范围内线性良好，ｒ＝０．９９９４。测定含双氯灭痛浓度为０．２０μｇ／ｍｉ的血浆样品，其批内（ｎ＝９）及批间（ｎ＝７）相对标准偏差分别为７．４％和７．６％，回收率为９１．３％～９３．２％。应用本法测定了１０名健康人口服双氯灭痛片剂后的体内血药浓度。Following a detailed study.a simple,rapid and accurate RP-HPLC method has been developed for the determination of diclofenac sodium in human plasma.The sample is separated isocratically within 7 min using an octadecyl-bonded silica column and a mobile phase of methanol and sodium acetate buffer(68:32.V/V.PH4.2).The compounds were quantitated using a ultraviolet detector operated at 274 nm which allowed determination of 0.10～2.50μg/ml of diclofenac sodium with high reproducibility.The limit of detection is 0.03μg/ml.Intraday and inter-day RSD for assaying the plasma sample containing0.20μg/ml concentration of diclofenac sodium were 7.4% (n=9)and 7.6%(n=7).respectively.The recoveries of diclofenac sodium were 91. 3% ～93. 2% for plasma.The method has been used to determine diclofenac sodium in plasma samples from ten volunteers and provide data on the pharmacokinetics of the drug.

关 键 词：双氯灭痛 高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学]