气相色谱法测定血浆中平痛新  

Determination of Nefopam in Plasma by Gas Chromatography

作　　者：陆兵[1] 石庭森[1] 

机构地区：[1]北京军事医学科学院毒物药物研究所

出　　处：《药物分析杂志》1995年第6期22-24,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：本实验以毛细管气相色谱法测定血浆中微量平痛新浓度，选用高选择性的氮磷检测器，以盐酸奥芬那君为内标。最低检测限可至５ｎｇ／ｍｌ血浆，药物浓度为２０～６００ｎｇ／ｍｌ血浆时，线性关系良好（ｒ＝０．９９９２）。含药２０．０、１００．０、４００．０ｎｇ／ｍｌ血浆时的分离度为４．１８±０．４４，平均回收率为（９８．０±８．１）％，平均日内和日间差异分别为４．７％和３．８％。本法可用于盐酸平痛新体内药物动力学与生物利用度研究。A GC/NPD method for the determination of nefopam in plasma was developed.Orphenadrine was used as internal standard for quantitation by relative peak area. The minimal detectable concentration of nefopam was 5 ng/ml plasma.The standard curve was linear in the concentration range from 20 to 600 ng/ml plasma(r=0.9992).The average recovery was(98.0±4.4)%.The intra-day and inter-day RSD was 4.7%and 3;8%respectively.The method could be applied for the studies of nefopam's pharmakinetics and bioavailabilit.

关 键 词：平痛新 气相色谱法 氮磷检测器 

分 类 号：R969.1[医药卫生—药理学]