硫酸长春地辛原料药纯度检验方法的研究  

Studies on Determination of the Purity of Vindesine Sulfate

作　　者：王劼 胡琴[1] 田南卉[1] 于魁一[1] 

机构地区：[1]北京市药品检验所

出　　处：《药物分析杂志》1995年第6期8-10,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：本文介绍了用ＴＬＣ和ＨＰＬＣ２种方法对硫酸长春地辛原料药进行纯度检验的实验。ＴＬＣ方法采用硅胶ＧＦ＿（２５４）为固定相，乙醚－甲醇－甲胺（２０：４：１）为展开剂，在紫外灯２５４ｎｍ下检视。ＨＰＬＣ法采用Ｃ＿（１８）化学键合硅胶为固定相，以０．０２ｍｏｌ／Ｌ磷酸二氢钾溶液（ｐＨ６．６）－甲醇（２０：８０）为流动相，在２６７ｎｍ波长下检测。结果表明，经优化条件后的ＴＬＣ和ＨＰＬＣ２种方法均可简便准确地检测硫酸长春地辛原料药中杂质含量，二者结果一致。TLC and HPLC methods were developed for the determination of the purity in vindesine sulfate,TLC method was,carried out by using silica gel F254 and ether-methanol-methylamine(20:4:1)as the mobile phase and UV detection at 254 nm. In HPLC analysis,analytical column was packed with C18-bonded silica gel,a solution of methanol and 0.02mol/L potassium dihydrogen phosphate (pH6. 6)(80:20)was employed as the mobile phase,the 267nm UV detector was selected.TLC and HPLC can determine the the purity in vindesine sulfate simply and pricisely under the optimized conditions.The results of the two methods are identical.

关 键 词：硫酸长春地辛 纯度检验 TLC法 HPLC法 

分 类 号：R927.12[医药卫生—药学]