高效液相色谱法测定人血舒林酸及其代谢物浓度  

HPLC Determination of Sulindac and Its Sulphone and Sulphide Metabolites in Serum

作　　者：王基平[1] 何小兵[1] 陈西敬[1] 熊冬生[1] 黄圣凯[1] 秦海敏[1] 

机构地区：[1]南京铁道医学院病理生理学教研室,中国药科大学药代动力学研究中心

出　　处：《药物分析杂志》1995年第5期7-9,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：本实验采用高效液相色谱法测定人血中舒林酸及其代谢物（硫化物、砜）的浓度。色谱柱为ＳｐｈｅｒｉｓｏｒｂＣ１８（５μ，４．６ｍｍ×２００ｍｍ）；流动相为乙腈－４％冰乙酸（５０：５０）５ｍｉｎ，乙腈－４％冰乙酸（８０：２０）１５ｍｉｎ；检测波长为３２８ｎｍ。采用吲哚美辛作为内标物，以峰高度定量。舒林酸、硫化物、砜的平均回收率分别为：（８２．７８±５．１５）％、（７４．１９±２．９３）％、（８５．８８±４．９１）％。日内及日间ＲＳＤ均＜１０％。本法适用于药代动力学和临床研究。A HPLC method for the determination of sulindac and its sulphone and sulphide metabolites in human serum was developed.The method utilized a Spherisorb C18 column,acetonitrile -0.4%acetic acid as mobile phase of an initial ratio of 50:50 for 5 min and then 80:20 for 15 min with a flow rate of 1ml/min,indomethacin as an internal standard.The absolute calibration curve(sulindac:C=0.5500H+0.0061,r=0.998,n=5:sulphone:C=.5760H+0.0836,r=0.9999,n=5;sulphide:C=0.4069H-0.0829,r=0.9999,n=5)was obtained with the range of 0.15～4. 8μg/ml.The average absolute recovery of sulindac,sulphone and sulphide from serum was(82.78±5.15)%,(74.19±2.93)%,(85. 88±4.91)%,respectively.The intraday and interday RSD were less than 10%.

关 键 词：高效液相色谱法 舒林酸 代谢产物 血液 

分 类 号：R969.1[医药卫生—药理学]