微生物法和荧光偏振免疫法测定丁胺卡那霉素血药浓度的相关性  

Comparative serum levels of AMIRACIN determined by microbiological and fluorescence polarization immunoassay methods

作　　者：赵爱国[1,2] 顾超宁 徐美娟 胡宁 

机构地区：[1]解放军第150医院药剂科,第二军医大学长征医院药械科 [2]海军药学研究中心,兰州军区后勤部药品检定所

出　　处：《药学实践杂志》1995年第4期231-234,共4页Journal of Pharmaceutical Practice

摘　　要：５例健康受试者肌内注射硫酸丁胺卡那霉素（４ｍｇ／Ｋｇ），１０４份血清样品分别经微生物法和荧光偏振免疫法测定丁胺卡那霉素血清药物浓度，结果表明荧光偏振免疫法测定结果显著高于微生物法的测定结果（Ｐ＜０．０１）。两组结果间具有良好的相关性，线性方程为：Ｙ＝１．７７５５＋０．９４９７Ｘ（γ＝０．９９６８），但微生物法在较低浓度时测定误差较大，精密度比荧光偏振免疫测定法低。本实验结果提示采用该两种方法均可对丁胺卡那霉素进行血药浓度监测。Serum levels of amikacin in 15 cases of healthy adult volunteers after intramuscular administration were determined by microbiological method (BA) and fluorescence polarization immunoassay method (FPIA).The result of all 104 serum samples showed that the serum levels of amikacin determined by FPIA method were significantly higher than those determined by the BA method(P<0. 01)the linear relation between these two groups was as follow:Y=1.7755+0.9497×(r=0. 9968).It suggested that monitoring the serum levet of amikacin by the two methods had no difference.

关 键 词：微生物法 丁胺卡那霉素 相关性 血药浓度 

分 类 号：R927.2[医药卫生—药学] R978.12