乌头碱对大鼠心肌细胞钙通道阻滞作用的单通道分析  

SINGLE CHANNEL ANALYSIS OF ACONITINE BLOCKADE OF CALCIUM CHANNELS IN RAT MYOCARDIOCYTES

作　　者：陈龙[1] 马骋[1] 蔡宝昌[1] 陆跃明 吴皓[1] 

机构地区：[1]南京中医学院中医药研究所

出　　处：《药学学报》1995年第3期168-171,共4页Acta Pharmaceutica Sinica

摘　　要：用膜片钳法，在培养的Ｗｉｓｔａｒ大鼠心室肌细胞上记录了Ｌ型钙通道的单通道活动。乌头碱５０μｇ·ｍｌ－１能显著阻断Ｌ型钙通道的活动，使其开放时间缩短、关闭时间延长、开放概率下降。与对照组比较，有显著性统计学差异（Ｐ＜０．０１）。而对通过Ｌ型钙通道的钡离于流幅值无明显影响。Ventricular myocardiocytes from neonatal Wistar rats were isolated and cultured.Aconitine,Ca2+ channel blocker verapamil or Ca2+ channel activator BAY K8644 were added to thebath solution separately. Using the cell-attached configuration of the patch clamp technique ,the singlechannel activities of L type Ca2+ channel were recorded before and after addition of all three drugs.The results showed the blocking effect of aconitine (50μg· ml-1)on L type Ca2+ channels. Itsmechanism may be relevant to the decrease in both open state probability and the mean open time ofCa2+ channel. The difference was statistically significant compared with control group(P<0.01).The amplitude of Ba2+ currents ,which flow through open L type Ca2+ channel was unchanged.

关 键 词：中药学 草乌 乌头碱 心肌细胞 膜片钳 钙通道 

分 类 号：R282.710.5[医药卫生—中药学]