番泻甙、大黄多糖和大黄素对脑细胞内游离钙浓度的影响  

EFFECTS OF SENNOSIDES,RHUBARB POLYSACCHARIDES AND EMODIN ON THE CYTOPLASMIC FREE CALCIUM IN ISOLATEC RAT BRAIN CELLS

作　　者：林秀珍[1] 靳珠华[1] 

机构地区：[1]天津医科大学药理研究室

出　　处：《药学学报》1995年第4期307-310,共4页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金

摘　　要：番泻甙、大黄多糖和大黄素对脑细胞内游离钙浓度的影响林秀珍，靳珠华（天津医科大学药理研究室３０００７０）近年来有关大黄及其有效成分的药理研究和临床应用有较大的进展［１，２］。但大黄有效成分番泻甙（ｓｅｎｎｏｓｉｄｅ，ＳＥＮ），大黄多糖（ｐｏｌｙｓａｃｃ...Free intracellular Ca2+([Ca2+]i) levels in rat brain were measured by Ca2+ sensitive fluorescent indicator Fura-2/Am and the effects of sennoside(SEN),polysaccharides fromRheum palmatum(RPP)and emodin(EMD)were studied.ResuIts showed that the resting[Ca2+]ilevel in the brain cells was l76. 25 ± 44.67 nmol·L-1(n=10)in Ca2+-free Hank’s solutioncontaining EGTA 0,5 mmol·L-1.After adding CaCl2(2 mmol·L-1)and KCI(120 mmol·L-1)to the brain cells suspension sequentially,the free[Ca2+]i levels were obviously elevated(P<0.01)compared with that of resting level.The brain cells were pretreated with SEN( 0.07,0.37mmol.L-1) for 10 min。 In the resting or using the above doses of CaCl2 and KCI,the[Ca2+]i wereobviously decreased compared with the control groups(P<0.01)at the same conditions.RPP dose-dependently decreased the [Ca2+]i.On the contrary,when brain cells were pretreated with EMD(0.037 mmol·L-1) for 10 min, the[Ca2+]i were obviously increased compared with the controlgroups(P<0.01)at the same conditions.The results showed that EMD could not only promote therelease of intracellular Ca2+ but also the influx of extracellular Ca2+, The opposite effects of thedifferent active components of rhubarb on the[Ca2+]i levels suggest that rhuharb may have differentkinds of regulatory functions on brain cells。

关 键 词：大黄 番泻甙 钙 实验药理 

分 类 号：R977.7[医药卫生—药品] R965.2[医药卫生—药学]