商陆多糖Ⅰ联合白细胞介素2对小鼠脾细胞杀瘤活性的影响  

EFFECTS OF PHYTOLACCA ACINOSA POLYSACCHARIDESI COMBLNED WITH INTERLEUKIN-O ON THE CYTOTOXICITY OF MURINE SPLENOCYTES AGAINST TUMOR CELLS

作　　者：王洪斌[1] 陈维中 包尔基[1] 郑钦岳[1] 宋梁 方军[1] 徐一新[1] 陈海生[1] 

机构地区：[1]上海第二军医大学药学院药理教研室,上海植物化学教研室,南京军区生物制品研究所,上海药品检验所

出　　处：《药学学报》1995年第6期401-407,共7页Acta Pharmaceutica Sinica

摘　　要：商陆多糖Ⅰ（ＰＡＰ－Ｉ），０．３～３μｇ·ｍｌ－１和小鼠脾细胞培养３～５ｄ可显著增强其杀伤Ｐ８１５肿瘤细胞活性及ＩＬ－２（２５０～５００ＩＵ·ｍｌ－１）诱导的ＬＡＫ细胞活性，最适浓度为１μｇ·ｍｌ－１。ＰＡＰ－Ｉ及ＩＬ－２和脾细胞培养的上清液对Ｐ８１５肿瘤细胞无细胞毒作用，但能增强脾细胞及ＬＡＫ细胞杀瘤活性。ＰＡＰ－Ｉ，５，１０及５０ｍｇ·ｋｇ－１，ｉｐ可增强脾细胞杀伤Ｐ８１５和Ｌ９２９细胞的活性及ＩＬ－２诱导的ＬＡＫ细胞活性。Phytolacca acinosaccharides I(PAP-I),a kind of purified polysac- charides,isolated from Phytolacca acinosa Roxb was found to significantly augment the cyto- toxicity of murine splenocytes and interleukin-2( IL-2)activated splenocytes against P815 tumor cells in uitro. The optimal concentration of PAP-I was 1μg·ml-1 and the peak level of the cytotoxicity against P815 tumor cells was reached on d 3～5.The supernatants collected from splenocytes cultured with PAP-I alone or in combination with IL-2 showed no effect on the cytotoxicity against P815 tumor cells.Splenocytes from mice injected ip with PAP-I,5, 10 and 50 mg·kg-1 ,thrice a week produced more cytotoxicity against P815 and L929 tumor cells compared with the control group.PAP-I ip was shown to significantly increase IL-2 activated killer cell activity(LAK)against P815 tumor cells.The higher the dosage of PAP-I,the more potent the LAK activity was observed.These results confirmed that PAP-I can augment the cytotoxicity of murine splenocyte against tumor cells and LAK activity and warranted further evaluation of its clinical usefulness.

关 键 词：商陆 多糖 白细胞介素2 MTT比色法 脾 肿瘤细胞 

分 类 号：R282.710.5[医药卫生—中药学] R73－361[医药卫生—中医学]