银杏叶中双黄酮成分的提取分离及其HPLC法测定  

EXTRACTION,ISOLATION AND HPLC DETERMINATION OF BIFLAVONES IN GINKGO BILOBAL

作　　者：钟郁青 徐礼 

机构地区：[1]中国医学科学院,中国协和医科大学药物研究所,北京医科大学

出　　处：《药学学报》1995年第9期694-697,共4页Acta Pharmaceutica Sinica

摘　　要：研究了银杏叶中所含３种双黄酮成分：银杏双黄酮、异银杏双黄酮及７－去甲基银杏双黄酮的提取、分离、纯化及用ＨＰＬＣ法测定生药中３种成分含量。Three kinds of biflavone(ginkgetin, isoginkgetin and bilobetin ) were isolatedfrom Ginkgko biloba L.and were determined by reversed phase HPLC using anthracene as internalstandard. The column employed was a 10 μm LiChrosorb RP-18(250 mm × 4 mm),the elutingsolvent consisted of methanol water formic acid(85∶15 ∶ 0. 8 υ/υ)and the effluent wasmonitored at 330 nm. The method is simple,rapid and accurate,the relative standard deviations ofthe three compounds are 0.996%,1.76%and 0.332%,respectively.

关 键 词：双黄酮 银杏 叶 高效液相色谱法 分离 提取 

分 类 号：R284.2[医药卫生—中药学] R282.710.3[医药卫生—中医学]