甲型肝炎减毒活疫苗的研究Ⅱ.实验性疫苗的研制  

The Trial Preparation of Attenuated Live Hepatitis A Vaccine

作　　者：董德祥[1] 毛江森[2] 陈统球[1] 练幼辉[1] 陈念良[2] 李靖[1] 柴少爱[1] 李华[1] 任丽萍[1] 黄海英[2] 谢汝英 阳选祥[1] 余哲[2] 余佩华[2] 曹逸云[1] 

机构地区：[1]中国医学科学院医学生物学研究所 [2]浙江省医学科学院微生物学研究所

出　　处：《中国医学科学院学报》1989年第1期1-6,共6页Acta Academiae Medicinae Sinicae

摘　　要：用H_2M_(20)K_5(32℃)甲型肝炎减毒株在KMB_(17)人胚肺二倍体细胞上制备一批实验性甲型肝炎减毒活疫苗。经暂定的生物制品检定项目检测,疫苗内不含外源因子,感染性滴度为10^(6.5)TCID_50/ml,疫苗病毒为HAV。12只猕猴体内试验表明,疫苗接种后不引起具临床重要意义的肝炎指征,但可使全部猕猴产生抗HAV抗体。本批疫苗的安全性和免疫原性在猕猴体内得到证实,有必要在少量人群中作进一步试用观察。The experimental attenuated live hepatitis A vaccine has been prepared in KMB17 human fetal lung diploid cells with the HAV variant H2M20K5 (32℃). No extraneous agent was detected in the vaccine, which had a titer of 106.5 TCID50/ml. Twelve monkeys vaccinated intravenously or subcuta-neously were found to have no significant clinical hepatitis but with anti-HAV antibody in all cases. Thus the safety and immunogenicity of the vaccine were evidenced in monkeys and it is necessary to investigate the trial vaccination further in a few susceptible individuals.

关 键 词：甲型肝炎 疫苗 减毒活疫苗 

分 类 号：R392－33[医药卫生—免疫学]