用RAPD方法分析水稻光敏核不育基因  

Tagging Rice Photoperiod-sensitive Genic Male Sterile Gene by RAPD Analysis

作　　者：王京兆[1] 王斌[1] 徐琼芳[1] 杨代常[1] 朱英国[1] 

机构地区：[1]中国科学院遗传研究所,武汉大学生物系

出　　处：《Acta Genetica Sinica》1995年第1期53-58,共6页

摘　　要：本文以农垦５８Ｓ与ＦＬ_２杂交所得的Ｆ_２分离群体为材料，按其育性分为不育和可育两大群体，分别以混合的可育群体核ＤＮＡ和不育群体核ＤＮＡ为模板，进行ＲＡＰＤ分析，从检测过的３００个引物中发现有２个引物在不育群体和可育群体中扩增出多态性产物。就其中一个引物对杂交亲本和Ｆ_２个体的ＲＡＰＤ分析进一步证明了这种多态的可靠性。转移杂交实验表明这个多态性产物是一种重复顺序。Bulked DNAs from F2 segregating population of the cross Nongken 58S×FL2 were used for RAPD analysis.300 primers used for the detection of polymorphism,two of them gave polymorphic amplification products.RAPD analysis of the parents and individuals of F2 segregating population with one primer OPX-07,also identified the consistancy of the polymorphic product.It is suggested that this amplified product is linked with the PGMS gene,but the distance is not known.Southern hybridization results indicate that the polymorphic product is a kind of repeated sequence.

关 键 词：光敏核不育基因 RAPD 水稻 

分 类 号：S511.103.2[农业科学—作物学]