用新的分子标记方法（RAPD）分析小麦抗白粉病基因Pm4α的近等基因系  

Tagging the Pm4α Gene in NILs by RAPD Analysis

作　　者：李松涛[1] 张忠廷[1] 王斌[1] 钟少斌[1] 姚景侠[1] 

机构地区：[1]中国科学院遗传研究所,江苏省农业科学院遗传生理研究所

出　　处：《Acta Genetica Sinica》1995年第2期103-108,共6页

摘　　要：ＲＡＰＤ是一种新发展的分子标记技术，本实验利用这种技术对小麦抗白粉病基因Ｐｍ４α的近等基因系进行分析。在１００个随机引物中找到了３个引物在这对抗白粉病的近等基因系中所扩增出的带型出现差异。并根据理论计算所找到的差异与抗性基因Ｐｍ４α连锁的概率是０．７，即３个差异中应有２个标记与抗性基因连锁。The powdery mildew resistant gene(Pm4α) is an important source in wheatbreeding. In order to tag the gene,the recently developed RAPD technique was utilized to identify molecular markers linked to Pm4α using NILs(near-isogenic lines).100 primers were screened by RAPD analysis,three of them amplified polymorphicDNA products.By theoretical calculation,the frequence of polymorphism betweenthe pair of NILs obtained after 7 generals backcrosses should be 4.5 polymorphicDNA fragments every 100 primers(in practice we got three). Two of the three obtained polymorphic fragments should be linked to Pm4α gene. The results are consistent with that obtained .Received July 12,1993

关 键 词：小麦白粉病 分子标记 抗白粉病基因 近等基因系 

分 类 号：S512.103.4[农业科学—作物学]