家兔HDL的纯化和鉴定及其功能的初步研究  

The Primary Study on Purification, Characterization and Function of Rabbit Serum HDL

作　　者：胡咏梅 薛红[1] 吴钢[1] 王克勤[1] 夏人仪[1] 赵三妹[1] 

机构地区：[1]中国医学科学院基础医学研究所

出　　处：《中国医学科学院学报》1989年第6期463-365,共1页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金

摘　　要：流行病学调查及动物实验结果均表明,高密度脂蛋白(HDL)具有抗动脉粥样硬化作用。作者为进一步了解HDL在细胞水平上的作用,建立了用密度梯度区带超速离心直接从新鲜家兔血清中分离纯化HDL的方法;将HDL加入正常及高脂血兔血清,观察低密度脂蛋白(LDL)通过平滑肌细胞(SMC)LDL受体的cpm,并分别与各自未加HDL组比较,以了解HDL在正常及高脂情况下对LDL受体的作用.Rabbit serum HDL was isolated by gradient ultracentrifugation, found to be pure on PAGE, and characterized by PAGIF. The effect of HDL on normal and hyperlipidemic uptake of LDL into SMC through LDL receptors in rabbits was observed in three experiments. The percentage decrease between normal groups with and without HDL was small (2.3%, 5.5%,7.3%), while the percentage decrease between hyperlipidemic groups with and without was large (23%, 25%, 26%). The results demonstrate that HDL affects the uptake of hyperlipidemic LDL into SMC through LDL receptors. This suggests that HDL might regulate the cholesterol overloading in cells in cases of hyperlipidemic LDL. The mechanism of HDL action is discussed.

关 键 词：脂蛋白 纯化 鉴定 兔 

分 类 号：R392－33[医药卫生—免疫学]