肝微粒体酶在集落形成试验中对环磷酰胺和甲基苄肼的激活作用  

ACTIVATION OF CYCLOPHOSPHAMIDE AND PROCARBAZINE BY LOW DOSE OF RAT LIVER MICROSOME ENZYMES IN TCFA

作　　者：刘全海[1] 李可[1] 顾春露 张慧娟[1] 

机构地区：[1]上海医药工业研究院,上海200433

出　　处：《中国医药工业杂志》1989年第8期356-359,共4页Chinese Journal of Pharmaceuticals

摘　　要：S-9 10μl/ml不减少肿瘤细胞集落形成。当其直接加入双层软琼脂系统上层,在环磷酰胺浓度为25,50,100和200μg/ml时,L_(1210)细胞的集落形成率分别为66,45,39和9％,HeLa细胞集落形成率为96,81,66和38％;甲基苄肼浓度为100,200和400μg/ml时,B_(16)细胞集落形成率为64,45和26％。不加S-9时,上述药物对各种细胞均不显示抑制作用。但人肝癌细胞株SMMC_(7721)虽不加S-9,在上述二药作用下集落形成率明显降低。S-9 40 μl/ml had serious toxicity on tumor cells, but 10μl/mldid not decrease plating efficiency of L_(1210), B_(16),HeLa and SMMC_(7721) cell lines intumor colony forming assay (TCFA).Cyclophosphamide at 25, 50, 100 and 200μg/ml with S-9 10μl/ml inhibited plating efficiency of L_(1210) to 66, 45, 39 and9%, and that of HeLa to 96, 81, 66 and 38%.Procarbazine at 100, 200 and 400μg/ml with S-9 10μl/ml reduced plating efficiency of B_(16) to 64, 45, and 26%.Both drugs at the above concentrations without S-9 did not inhibit colony formingof L-(1210), B_(16) and HeLa cells, but significantly inhibited plating efficiency ofSMMC_(7721).

关 键 词：微粒体酶 环磷酰胺 甲基苄肼 肿瘤 

分 类 号：R969.4[医药卫生—药理学]